The Relapsing Fever Spirochete Borrelia hermsii Contains Multiple, Antigen-Encoding Circular Plasmids That Are Homologous to the cp32 Plasmids of Lyme Disease Spirochetes
| Autor: | Merry E. Schrumpf, Stephen F. Porcella, Katrina L. Oie, Brian Stevenson, Sandra J. Raffel, Lori Lubke, Tom G. Schwan, Cecily A. Fitzpatrick |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial relapsing fever Immunology Molecular Sequence Data Spirochaetaceae Microbiology Open Reading Frames Plasmid Lyme disease Borrelia burgdorferi Group Species Specificity medicine Animals Humans Amino Acid Sequence Borrelia burgdorferi Gene Antigens Bacterial biology Base Sequence Sequence Homology Amino Acid Borrelia Hemolysin medicine.disease biology.organism_classification bacterial infections and mycoses Virology Infectious Diseases Genes Bacterial Molecular and Cellular Pathogenesis Parasitology Borrelia hermsii DNA Probes Plasmids |
| Popis: | Borrelia hermsii , an agent of tick-borne relapsing fever, was found to contain multiple circular plasmids approximately 30 kb in size. Sequencing of a DNA library constructed from circular plasmid fragments enabled assembly of a composite DNA sequence that is homologous to the cp32 plasmid family of the Lyme disease spirochete, B. burgdorferi . Analysis of another relapsing fever bacterium, B. parkeri , indicated that it contains linear homologs of the B. hermsii and B. burgdorferi cp32 plasmids. The B. hermsii cp32 plasmids encode homologs of the B. burgdorferi Mlp and Bdr antigenic proteins and BlyA/BlyB putative hemolysins, but homologs of B. burgdorferi erp genes were absent. Immunoblot analyses demonstrated that relapsing fever patients produced antibodies to Mlp proteins, indicating that those proteins are synthesized by the spirochetes during human infection. Conservation of cp32-encoded genes in different Borrelia species suggests that their protein products serve functions essential to both relapsing fever and Lyme disease spirochetes. Relapsing fever borreliae replicate to high levels in the blood of infected animals, permitting direct detection and possible functional studies of Mlp, Bdr, BlyA/BlyB, and other cp32-encoded proteins in vivo. |
| Databáze: | OpenAIRE |
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