A native plasmid of Pasteurella haemolytica serotype A1: DNA sequence analysis and investigation of its potential as a vector
| Autor: | G. D. Baird, F. A. Lainson, F Wright, A.R. Wood, W Donachie |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Electrophoresis
Agar Gel ColE1 General Veterinary Base Sequence Sequence analysis animal diseases Mutant Genetic Vectors Molecular Sequence Data Nucleic acid sequence Sequence Analysis DNA Biology medicine.disease_cause Microbiology Plasmid Shuttle vector medicine DNA Transposable Elements bacteria Insertion sequence Serotyping Escherichia coli Mannheimia haemolytica Plasmids |
| Zdroj: | Research in veterinary science. 58(2) |
| ISSN: | 0034-5288 |
| Popis: | The complete nucleotide sequence of a 4·3 kilobase pair plasmid, pAB2, isolated from a bovine strain of Pasteurella haemolytica serotype Al, was determined. It encodes a Rob-1 type β-lactamase and a region with homology to the mobilisation ( mob ) region of the Escherichia coli plasmid, ColE1. An insertion mutant of pAB2 (pTC2/81) carrying a copy of Tn5 was transferred to E coli K12 by conjugation. Subsequently pTC2/81 could be transferred by transformation to E coli HB101, but not to P haemolytica serotypes Al or A2. However, a derivative of this construct containing only a fragment of the Tn5 insertion sequence was able to transform P haemolytica. A further construct containing a fragment of the P haemolytica Al leucotoxin A gene, was similarly restricted to transforming E coli . These results demonstrate that the pAB2 plasmid is capable of acting as an E coli/P haemolytica shuttle vector. However, the nature of the cloned DNA sequences are important to transformation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect