Cold storage (8 C) of islets of Langerhans in a high-potassium 'intracellular medium': near-normal insulin release and morphology after one week
| Autor: | N. R. Norlund, B. J. Frankel |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Potassium chemistry.chemical_element Cold storage Mice Obese Biology Tissue culture Islets of Langerhans Mice Endocrinology Internal medicine Culture Techniques Insulin Secretion medicine Animals Insulin Fetus geography geography.geographical_feature_category Islet In vitro Culture Media Cold Temperature chemistry Female Tissue Preservation Intracellular |
| Zdroj: | Journal of endocrinological investigation. 8(1) |
| ISSN: | 0391-4097 |
| Popis: | Islets of Langerhans from ob/ob mice were microdissected and stored for one week at 8 C or 37 C in a high-potassium "intracellular medium" or in a traditional culture medium (RPMI 1640 +/- fetal calf serum). Subsequent glucose-stimulated insulin release and islet morphology were best preserved by 8 C-storage in the high-potassium "intracellular medium". This suggests that normal, adult, mammalian tissues which do not readily divide in vitro may be better preserved for short periods of time at refrigerator temperatures in a high-potassium medium than by conventional culture at 37 C in a tissue culture medium. |
| Databáze: | OpenAIRE |
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