Brain incorporation of [11C]arachidonic acid in young healthy humans measured with positron emission tomography
| Autor: | Ying Ma, Michael A. Channing, Stanley I. Rapoport, Alicja Mason, Michael C. J. Chang, Catherine Connolly, Arun L.W. Bokde, William C. Eckelman, Richard E. Carson, Margaret G. Der, Bik-Kee Vuong, Peter Herscovitch, Maria T. Toczek, Doris J. Doudet, Giampiero Giovacchini |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Models Biological Second Messenger Systems Phospholipases A 030218 nuclear medicine & medical imaging 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phospholipase A2 In vivo medicine Humans Carbon Radioisotopes Phospholipids chemistry.chemical_classification Arachidonic Acid biology medicine.diagnostic_test Chemistry Brain Metabolism Kinetics Phospholipases A2 Enzyme Neurology Biochemistry Positron emission tomography Second messenger system biology.protein Arachidonic acid Neurology (clinical) Signal transduction Cardiology and Cardiovascular Medicine 030217 neurology & neurosurgery Tomography Emission-Computed |
| Zdroj: | Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 22(12) |
| ISSN: | 0271-678X |
| Popis: | Arachidonic acid (AA) is an important second messenger involved in signal transduction mediated by phospholipase A2. The goal of this study was to establish an in vivo quantitative method to examine the role of AA in this signaling process in the human brain. A simple irreversible uptake model was derived from rat studies and modified for positron emission tomography (PET) to quantify the incorporation rate K*of [11C]AA into brain. Dynamic 60-minute three-dimensional scans and arterial input functions were acquired in 8 young healthy adults studied at rest. Brain radioactivity was corrected for uptake of the metabolite [11C]CO2. K* and cerebral blood volume ( Vb) were estimated pixel-by-pixel and were calculated in regions of interest. K* equaled 5.6 ± 1.2 and 2.6 ± 0.5 μL · min−1· mL−1in gray and white matter, respectively. K* and Vbvalues were found to be unchanged with data analysis periods from 20 to 60 minutes. Thus, PET can be used to obtain quantitative images of the incorporation rate K* of [11C]AA in the human brain. As brain incorporation of labeled AA has been shown in awake rats to be increased by pharmacological activation associated with phospholipase A2-signaling, PET and [11C]AA may be useful to measure signal transduction in the human brain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|