Responses of marine mussel Mytilus galloprovincialis (Bivalvia: Mytilidae) after infection with the pathogen Vibrio splendidus

Autor: Mylène Toubiana, Tiziana Cappello, Angela Mauceri, Maria Maisano, Matteo Cammarata, Maria Giovanna Parisi, Sabrina Oliva
Přispěvatelé: Parisi, Maria Giovanna, Maisano, Maria, Cappello, Tiziana, Oliva, Sabrina, Mauceri, Angela, Toubiana, Mylene, Cammarata, Matteo, Hydrosciences Montpellier (HSM), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Muscle tissue
animal structures
Physiology
Health
Toxicology and Mutagenesis
[SDV]Life Sciences [q-bio]
Osmotic balance
Bivalve molluscs
Cell turnover
Hemolymph
In vivo infection
Pathogenic bacteria
Posterior adductor muscle
Toxicology
medicine.disease_cause
01 natural sciences
Biochemistry
[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity
Microbiology
03 medical and health sciences
Immune system
medicine
Animals
14. Life underwater
Bivalve mollusc
Vibrio
Mytilus
biology
010604 marine biology & hydrobiology
fungi
Cell Biology
General Medicine
Mussel
Water-Electrolyte Balance
biology.organism_classification
Bivalvia
030104 developmental biology
medicine.anatomical_structure
Mytilidae
13. Climate action
Host-Pathogen Interactions
[SDV.IMM]Life Sciences [q-bio]/Immunology
Zdroj: Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology
Comparative Biochemistry and Physiology-Part C: Toxicology and Pharmacology, Elsevier, 2019, 221, pp.1-9. ⟨10.1016/j.cbpc.2019.03.005⟩
ISSN: 1532-0456
Popis: International audience; Bivalve molluscs possess effective cellular and humoral defence mechanisms against bacterial infection. Although the immune responses of mussels to challenge with pathogenic vibrios have been largely investigated, the effects at the site of injection at the tissue level have not been so far evaluated. To this aim, mussels Mytilus galloprovincialis were herein in vivo challenged with Vibrio splendidus to assess the responses induced in hemolymph and posterior adductor muscle (PAM), being the site of bacterial infection. The number of living intra-hemocyte bacteria increased after the first hour post-injection (p.i.), suggesting the occurrence of an intense phagocytosis, while clearance was observed within 24 h p.i. A recruitment of hemocytes at the injection site was found in mussel PAM, together with marked morphological changes in the volume of muscular fibers, with a recovery of muscle tissue organization after 48 h p.i. A concomitant impairment in the osmoregulatory processes were observed in PAM by an initial inhibition of aquaporins and increased immunopositivity of Na+/K+ ATPase ionic pump, strictly related to the histological alterations and hemocyte infiltration detected in PAM. Accordingly, an intense cell turnover activity was also recorded following the infection event. Overall, results indicated the hemolymph as the system responsible for the physiological adaptations in mussels to stressful factors, such as pathogenicity, for the maintenance of homeostasis and immune defence. Also, the osmotic balance and cell turnover can be used as objective diagnostic criteria to evaluate the physiological state of mussels following bacterial infection, which may be relevant in aquaculture and biomonitoring studies.
Databáze: OpenAIRE
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