Mutated beta-catenin evades a microRNA-dependent regulatory loop
| Autor: | Luigi Bolondi, Laura Lupini, Francesca Lovat, Laura Gramantieri, Mario Acunzo, Taewan Kim, Veronica Balatti, Jessica Consiglio, Angelo Veronese, Carlo M. Croce, Danilo Perrotti, Manuela Ferracin, Rosa Visone, Lucilla D'Abundo, Yuri Pekarsky, Massimo Negrini, Elena Miotto |
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| Přispěvatelé: | Veronese A, Visone R, Consiglio J, Acunzo M, Lupini L, Kim T, Ferracin M, Lovat F, Miotto E, Balatti V, D'Abundo L, Gramantieri L, Bolondi L, Pekarsky Y, Perrotti D, Negrini M, Croce CM. |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Beta-catenin
endocrine system diseases BETA-CATENIN NO microRNA beta catenin cancer Insulin-Like Growth Factor II Cell Line Tumor Proto-Oncogene Proteins Puma microRNA Humans cancer Gene Transcription factor Multidisciplinary biology HEK 293 cells Intron Biological Sciences biology.organism_classification Introns MICRO-RNA MicroRNAs HEK293 Cells Genetic Loci Catenin Mutation Cancer research biology.protein beta catenin Apoptosis Regulatory Proteins Transcription Factors |
| Popis: | hsa-mir-483 is located within intron 2 of the IGF2 gene. We have previously shown oncogenic features of miR-483-3p through cooperation with IGF2 or by independently targeting the proapoptotic gene BBC3/PUMA. Here we demonstrate that expression of miR-483 can be induced independently of IGF2 by the oncoprotein β-catenin through an interaction with the basic helix–loop–helix protein upstream stimulatory transcription factor 1. We also show that β-catenin itself is a target of miR-483-3p, triggering a negative regulatory loop that becomes ineffective in cells harboring an activating mutation of β-catenin. These results provide insights into the complex regulation of the IGF2/miR-483 locus, revealing players in the β-catenin pathway. |
| Databáze: | OpenAIRE |
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