Treatment of Swine Closed Head Injury with Perfluorocarbon NVX-428
| Autor: | Ashraful Haque, Charles R. Auker, Brittany Hazzard, Francoise Arnaud, Anke H. Scultetus, Paula F. Moon-Massat, Maj Erin Morris, Lam Thuy Vi Tran Ho, Saha Biswajit, Richard M. McCarron |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Resuscitation
oxygen therapeutic perfluorocarbon Traumatic brain injury business.industry medicine.medical_treatment lcsh:R lcsh:Medicine Brain damage Oxygenation medicine.disease Polytrauma Article Anesthesia Closed head injury medicine neuroprotection brain trauma medicine.symptom business dodecafluoropentane Saline Spongiosis |
| Zdroj: | Medical Sciences Volume 8 Issue 4 Medical Sciences, Vol 8, Iss 41, p 41 (2020) |
| ISSN: | 2076-3271 |
| DOI: | 10.3390/medsci8040041 |
| Popis: | Pre-hospital treatment of traumatic brain injury (TBI) with co-existing polytrauma is complicated by requirements for intravenous fluid volume vs. hypotensive resuscitation. A low volume, small particle-size-oxygen-carrier perfluorocarbon emulsion NVX-428 (dodecafluoropentane emulsion 2% w/v) could improve brain tissue with minimal additional fluid volume. This study examined whether the oxygen-carrier NVX-428 shows safety and efficacy for pre-hospital treatment of TBI. Anesthetized swine underwent fluid percussion injury TBI and received 1 mL/kg IV NVX-428 (TBI-NVX) at 15 min (T15) or normal saline (no-treatment) (TBI-NON). Similarly, uninjured swine received NVX-428 (SHAM-NVX) or normal saline (SHAM-NON). Animals were monitored and measurements were taken for physiological and neurological parameters before euthanasia at the six-hour mark (T360). Histopathological analysis was performed on paraffin embedded tissues. Physiological, biochemical and blood gas parameters were not different, with the exception of a significant but transient increase in mean pulmonary artery pressure observed in the TBI-experimental group immediately after drug administration. There were no initial differences in brain oxygenation at baseline, but over time oxygen decreased ~50% in both TBI groups. Histological brain injury scores were similar between TBI-NVX and TBI-NON, although a number of subcategories (spongiosis-ischemic/dead neurons-hemorrhage-edema) in TBI-NVX had a tendency for lower scores. The cerebellum showed significantly lower spongiosis and ischemic/dead neuron injury scores and a lower number of Fluoro-Jade-B-positive cerebellar-Purkinje-cells after NVX-428 treatment compared to controls. NVX-428 may assist in mitigating secondary cellular brain damage. |
| Databáze: | OpenAIRE |
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