GAP-43 (B-50) and C-Jun Are Up-Regulated in Axotomized Neurons of Clarke's Nucleus after Spinal Cord Injury in the Adult Rat
| Autor: | Johannes Noth, A. B. Schmitt, Ch. Spitzer, Georg W. Kreutzberg, W. Nacimiento, M. Voell, Katrin Pech, Sebastian Breuer, Franz-Werner Schwaiger, Gary A. Brook |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Nervous system
Proto-Oncogene Proteins c-jun medicine.medical_treatment Central nervous system Biology lcsh:RC321-571 Rats Sprague-Dawley GAP-43 Protein medicine Animals RNA Messenger Phosphorylation AP-1-complex lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Spinal cord injury In Situ Hybridization Spinal Cord Injuries Neurons phospho-C-Jun Axotomy RNA Probes Spinal cord medicine.disease Immunohistochemistry Neuroregeneration spinal cord injury Rats Up-Regulation Cell biology medicine.anatomical_structure Spinal Cord Neurology Peripheral nervous system Female Neuroscience Nucleus signal transduction |
| Zdroj: | Neurobiology of Disease, Vol 6, Iss 2, Pp 122-130 (1999) |
| ISSN: | 0969-9961 |
| DOI: | 10.1006/nbdi.1998.0231 |
| Popis: | The growth-associated protein GAP-43 (B-50) and the transcription factor C-Jun are involved in regeneration of the injured nervous system. In this study, we investigated the possibility of the induction of GAP-43 and C-Jun in axotomized neurons of Clarke's nucleus (CN) in adult rats, of which a large population undergoes degeneration several weeks after a low thoracic lateral funiculotomy of the spinal cord. In situ hybridization and immunohistochemistry revealed a transient up-regulation of GAP-43 mRNA, C-Jun protein, and its activated, phosphorylated form, peaking around 7 days after injury in preferentially large diameter CN-neurons ipsilateral and caudal to the lesion. Our results document that some populations of axotomized central nervous system neurons, similar to axotomized regenerating neurons of the peripheral nervous system, can up-regulate GAP-43 and C-Jun, even if they are destined to degenerate. This might reflect a transient regenerative capacity, which fails over time. |
| Databáze: | OpenAIRE |
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