Oxidative stress and pro-apoptotic conditions in a rodent model of Wilson's disease
| Autor: | Marie Therese Armentero, Anna Mangiagalli, Plinio Richelmi, Andrea Ferrigno, Eleonora Bazzini, R. Fancellu, Fabio Blandini, Giuseppe Nappi, Mariapia Vairetti, Alberta Samuele |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Lipid Peroxides
medicine.medical_specialty Peroxidation Cell degeneration Copper-toxicosis Apoptosis Striatum Nitric Oxide medicine.disease_cause Thiobarbituric Acid Reactive Substances Nitric oxide chemistry.chemical_compound Hepatolenticular Degeneration Internal medicine Cortex (anatomy) medicine Animals Biogenic Monoamines Sulfhydryl Compounds Long Evans Cinnamon Molecular Biology Chromatography High Pressure Liquid Transaminases Hepatitis Chronic Rats Inbred LEC Analysis of Variance Caspase 3 Superoxide Dismutase Brain Glutathione medicine.disease Rats Wilson's disease Oxidative Stress medicine.anatomical_structure Endocrinology Liver chemistry Cerebral cortex Caspases Molecular Medicine apoptosis cell degeneration copper-toxicosis long evans cinnamon peroxidation Oxidative stress |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1741:325-330 |
| ISSN: | 0925-4439 |
| DOI: | 10.1016/j.bbadis.2005.06.004 |
| Popis: | Wilson's disease (WD) is an inherited disorder, characterized by selective copper deposition in liver and brain, chronic hepatitis and extra-pyramidal signs. In this study, we investigated changes of biochemical markers of oxidative stress and apoptosis in liver, striatum and cerebral cortex homogenates from Long–Evans Cinnamon (LEC) rats, a mutant strain isolated from Long Evans (LE) rats, in whom spontaneous hepatitis develops shortly after birth. LEC and control (LE) rats at 11 and 14 weeks of age were used. We determined tissue levels of glutathione (GSH/GSSG ratio), lipid peroxides, protein-thiols (P-SH), nitric oxide metabolites, activities of caspase-3 and total superoxide-dismutase (SOD), striatal levels of monoamines and serum levels of hepatic amino-transferases. We observed a decrease of protein-thiols, GSH/GSSG ratio and nitrogen species associated to increased lipid peroxidation in the liver and striatum – but not in the cerebral cortex – of LEC rats, accompanied by dramatic increase in serum amino-transferases and decrease of striatal catecholamines. Conversely, SOD and caspase-3 activity increased consistently only in the cortex of LEC rats. Hence, we assume that enhanced oxidative stress may play a central role in the cell degeneration in WD, at the main sites of copper deposition, with discrete pro-apoptotic conditions developing in distal areas. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect