Suitability of teicoplanin-aglycone bonded stationary phase for simulated moving bed enantio separation of racemic amino acids employing composition-constrained eluents
| Autor: | Matthias Bechtold, Sven Panke, Matthias Heinemann |
|---|---|
| Přispěvatelé: | Molecular Systems Biology |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
COMPETITIVE ADSORPTION-ISOTHERMS
ORGANIC-SOLVENTS mobile phase composition model-based experimental analysis Analytical chemistry column stability MACROCYCLIC ANTIBIOTICS Biochemistry High-performance liquid chromatography teicoplanin-aglycone simulated moving bed modeling Analytical Chemistry chemistry.chemical_compound symbols.namesake Adsorption DISPERSION Desorption RETENTION Amino Acids Chromatography High Pressure Liquid Aqueous solution Chromatography STABILITY Elution enantioseparation of amino acids Organic Chemistry Langmuir adsorption model MIXTURES Stereoisomerism General Medicine Hydrogen-Ion Concentration chemistry SELECTIVITY RESOLUTION symbols Thermodynamics Simulated moving bed Enantiomer Teicoplanin LIQUID-CHROMATOGRAPHY |
| Zdroj: | Journal of Chromatography A, 1113(1-2), 167-176. ELSEVIER SCIENCE BV |
| ISSN: | 0021-9673 |
| Popis: | The suitability of a teicoplanin-aglycone based chiral stationary phase for the simulated moving bed (SMB) enantioseparation of amino acids under enzyme-compatible conditions was shown following a procedure that is based solely on model-based simulations and HPLC experiments. A set of eight amino acids could be separated employing aqueous solvent containing only 10% (v/v) methanol, five of them with baseline resolution. The impact of type and concentration of organic modifier and pH modifier and pH on the separation characteristics of racemic methionine was investigated. Invariant elution profiles of repetitive adsorption/desorption of large amounts of methionine representing SMB-like conditions suggest stable adsorption behavior. Competitive loading capacity (20 mg of methionine per g of chiral stationary phase (CSP)) and SMB productivity (1 g Of D-methionine per g of CSP per day) were predicted. The applied transport-dispersive model based on a competitive Bi-Langmuir isotherm was validated and its parameter estimated by model-based experimental analysis. (c) 2006 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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