Partial Characterization of Somatomedin Bioactivity in Term Human Amniotic Fluid
| Autor: | Glen R. Smith, R. Marvin Bala |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
medicine.medical_specialty
Amniotic fluid Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Size-exclusion chromatography Ultrafiltration Biochemistry Endocrinology Pregnancy Somatomedins Internal medicine medicine Animals Humans Bioassay Chromatography Chemistry Insulin Biochemistry (medical) Amniotic Fluid Somatomedin Rats Starch gel electrophoresis Ultrafiltration (renal) Cartilage Sephadex Biological Assay Female |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 43:907-912 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem-43-4-907 |
| Popis: | Somatomedin (SM) activity in human amniotic fluid (AF) was partially characterized. Aliquots of pooled AF, obtained at term gestation, were acidified to pH 2.3 and sequentially studied by ultrafiltration on various membranes, Sephadex gel filtration, and starch gel electrophoresis (SGE). SM activity was measured by a rat cartilage bioassay. SM activity in AF, under these conditions, was present in several stable indicated molecular size (IMS) forms; large (greater than 45,000) and small (near 10,000). The small SM was found in basic and acidic forms after SGE. These studies suggest that SM activity in term human AF is somewhat similar to SM in unextracted plasma. In addition, immunoreactive GH and insulin were measured in AF but at lower concentrations than usually found in plasma. After chromatography of AF, GH was found in large and small IMS forms, whereas most of the insulin was found in the monomer insulin IMS area. |
| Databáze: | OpenAIRE |
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