Enzymatic synthesis of novel oligosaccharides from N-acetylsucrosamine and melibiose using Aspergillus niger α-galactosidase, and properties of the products
| Autor: | Moe Katou, Mitsuru Tashiro, Yohei Sakaki, Chiseko Sakuma, Takako Hirano, Toshiyuki Nishio, Wataru Hakamata |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oligosaccharides Saccharomyces cerevisiae 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Hydrolysis Raffinose Melibiose Molecular Biology chemistry.chemical_classification Alpha-galactosidase biology beta-Fructofuranosidase 010405 organic chemistry Organic Chemistry Aspergillus niger General Medicine Oligosaccharide biology.organism_classification 0104 chemical sciences 030104 developmental biology Enzyme Invertase chemistry alpha-Galactosidase biology.protein Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 80(9) |
| ISSN: | 1347-6947 |
| Popis: | Two kinds of oligosaccharides, N-acetylraffinosamine (RafNAc) and N-acetylplanteosamine (PlaNAc), were synthesized from N-acetylsucrosamine and melibiose using the transgalactosylation activity of Aspergillus niger α-galactosidase. RafNAc and PlaNAc are novel trisaccharides in which d-glucopyranose residues in raffinose (Raf) and planteose are replaced with N-acetyl-d-glucosamine. These trisaccharides were more stable in acidic solution than Raf. RafNAc was hydrolyzed more rapidly than Raf by α-galactosidase of green coffee bean. In contrast, RafNAc was not hydrolyzed by Saccharomyces cerevisiae invertase, although Raf was hydrolyzed well by this enzyme. These results indicate that the physicochemical properties and steric structure of RafNAc differ considerably from those of Raf. Two kinds of novel trisaccharides were synthesized from N-acetylsucrosamine and melibiose, using transgalactosylation action of Aspergillus niger α-galactosidase. |
| Databáze: | OpenAIRE |
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