AMPK, a metabolic sensor, is involved in isoeugenol-induced glucose uptake in muscle cells
| Autor: | Chul Su Lee, Sun Hwa Park, Hyeon Soo Kim, Yong Woo Lee, Jung Ok Lee, Sun Woo Ryoo, Hye Jeong Lee, Hyung Ip Kim, Geum-Sook Hwang, Su Jin Kim, Nami Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
AMPK medicine.medical_specialty Endocrinology Diabetes and Metabolism Glucose uptake Muscle Fibers Skeletal Calcium-Calmodulin-Dependent Protein Kinase Kinase p38 MAPK AMP-Activated Protein Kinases Transfection p38 Mitogen-Activated Protein Kinases 03 medical and health sciences chemistry.chemical_compound Mice Endocrinology AMP-activated protein kinase Internal medicine Eugenol medicine Animals Phosphorylation RNA Small Interfering Protein kinase A Protein kinase B Cells Cultured Glucose Transporter Type 4 biology Research GTPase-Activating Proteins Glucose transporter glucose uptake Rats Enzyme Activation Isoeugenol 030104 developmental biology Glucose Biochemistry chemistry isoeugenol biology.protein Calcium GLUT4 |
| Zdroj: | The Journal of Endocrinology |
| ISSN: | 1479-6805 0022-0795 |
| Popis: | Isoeugenol exerts various beneficial effects on human health. However, the mechanisms underlying these effects are poorly understood. In this study, we observed that isoeugenol activated AMP-activated protein kinase (AMPK) and increased glucose uptake in rat L6 myotubes. Isoeugenol-induced increase in intracellular calcium concentration and glucose uptake was inhibited by STO-609, an inhibitor of calcium/calmodulin-dependent protein kinase kinase (CaMKK). Isoeugenol also increased the phosphorylation of protein kinase C-α (PKCα). Chelation of calcium with BAPTA-AM blocked isoeugenol-induced AMPK phosphorylation and glucose uptake. Isoeugenol stimulated p38MAPK phosphorylation that was inhibited after pretreatment with compound C, an AMPK inhibitor. Isoeugenol also increased glucose transporter type 4 (GLUT4) expression and its translocation to the plasma membrane. GLUT4 translocation was not observed after the inhibition of AMPK and CaMKK. In addition, isoeugenol activated the Akt substrate 160 (AS160) pathway, which is downstream of the p38MAPK pathway. Knockdown of the gene encoding AS160 inhibited isoeugenol-induced glucose uptake. Together, these results indicate that isoeugenol exerts beneficial health effects by activating the AMPK/p38MAPK/AS160 pathways in skeletal muscle. |
| Databáze: | OpenAIRE |
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