Cyanobacterium Synechocystis sp. PCC 6803 lacking adc1 gene produces higher polyhydroxybutyrate accumulation under modified nutrients of acetate supplementation and nitrogen-phosphorus starvation
| Autor: | Aran Incharoensakdi, Suthira Utharn, Panutda Yodsang, Saowarath Jantaro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Mutant
TCA tricarboxylic acid macromolecular substances DMF N N-dimethylformamide TAE Tris-acetate-ethylene diamine tetraacetic acid Applied Microbiology and Biotechnology Article HPLC high pressure liquid chromatography Polyhydroxybutyrate Pigment PCR polymerase chain reaction h hour(s) Synechocystis sp. PCC6803 Gene Carotenoid Nutrient deprivation chemistry.chemical_classification Chemistry Wild type ADC arginine decarboxylase PHB polyhydroxybutyrate DCW dry cell weight Biochemistry visual_art visual_art.visual_art_medium Adc1 mutant Arginine decarboxylase PHAs polyhydroxyalkanoates Intracellular TP248.13-248.65 Biotechnology |
| Zdroj: | Biotechnology Reports Biotechnology Reports, Vol 31, Iss, Pp e00661-(2021) |
| ISSN: | 2215-017X |
| Popis: | Highlights • The adc1 disruption of Synechocystis sp. PCC 6803 did not inhibit cell growth, as evidently shown by its similar growth with wild type, as well as its intracellular pigments. • The Δadc1 mutant has the highest capacity to produce PHB up to 36.1 %w/DCW under nitrogen and phosphorus-deprived medium containing acetate (BG11-N-P+A). • Abundant PHB granules stained by Nile-Red are markedly visualized in Δadc1 mutant under BG11-N-P and BG11-N-P+A when compared to those of wild type. • Transcript levels of phaA and phaB are mainly responsible to PHB content whereas the changed proportion of phaC and phaE is noted when a certain phaC transcript level is decreased. Increased polyhydroxybutyrate production in cyanobacterium Synechocystis sp. PCC 6803 lacking adc1 gene (Δadc1) is first-timely reported in this study. We constructed the mutant by disrupting adc1 gene encoding arginine decarboxylase, thereby exhibiting a partial blockade of polyamine synthesis. This Δadc1 mutant had a proliferative growth and certain contents of intracellular pigments including chlorophyll a and carotenoids as similar as those of wild type (WT). Highest PHB production was certainly induced by BG11-N-P+A condition in both WT and Δadc1 mutant of about 24.9 %w/DCW at day 9 and 36.1 %w/DCW at day 7 of adaptation time, respectively. Abundant PHB granules were also visualized under both BG11-N-P and BG11-N-P+A conditions. All pha transcript amounts of Δadc1 mutant grown at 7 days-adaptation time were clearly upregulated corresponding to its PHB content under BG11-N-P+A condition. Our finding indicated that this adc1 perturbation is alternatively achieved for PHB production in Synechocystis sp. PCC 6803. |
| Databáze: | OpenAIRE |
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