On the relationship of CSF pleocytosis to immunoglobulin levels as estimated by different techniques
| Autor: | Ronald C. Shortman, Jay Jethwa, Linda M. Luxon, Elizabeth Paul, Edward J. Thompson |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Electrophoresis
Immunology Immunoglobulins Immunoglobulin light chain chemistry.chemical_compound Leukocyte Count CSF pleocytosis Immunology and Allergy Lymphocytes Polyacrylamide gel electrophoresis Cerebrospinal Fluid Radial immunodiffusion Chromatography biology Chemistry Dansyl chloride Cerebrospinal Fluid Proteins Precipitin Tests Neurology Immunoglobulin G biology.protein Electrophoresis Polyacrylamide Gel Neurology (clinical) Antibody Densitometry Immunoglobulin Heavy Chains |
| Zdroj: | Journal of neuroimmunology. 2(3-4) |
| ISSN: | 0165-5728 |
| Popis: | One might expect a positive correlation between the number of CSF lymphocytes and the relative amount of CSF IgG, especially in cases of local synthesis of IgG within the CNS. However, previous attempts at correlating the number of CSF lymphocytes with IgG levels have yielded varying results. Since it is known that the estimation of CSF IgG levels can vary according to the method of immunoglobulin determination used, we decided to perform the analyses by three separate methods: (1) the commonly used, immunoprecipitation technique of radial immunodiffusion; (2) physico-chemical separation on the basis of hydrated size/charge using alkaline electrophoresis through the molecular sieving effects of polyacrylamide gels, followed by densitometry of the gamma region of Coomassie Blue-stained proteins; (3) separation into constituent Ig heavy chains and light chains on the basis of unfolded chain lengths by molecular sieving using sodium dodecyl sulphate (SDS), followed by densitometry of heavy chains of immunoglobulins having been labelled with the fluorescent dye dansyl chloride. Our results show a correlation of white count with the latter two techniques but not the former. Possible reasons for discrepancies in the literature are discussed with regard to the peculiar physical properties of the selected CSF IgG molecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|