Molecular Analysis of Oral Bacteria in Heart Valve of Patients With Cardiovascular Disease by Real-Time Polymerase Chain Reaction
| Autor: | Carlos Roberto Martins Rodrigues Sobrinho, Mário Rogério Lima Mota, Raquel Carvalho Montenegro, Francisco Artur Forte Oliveira, Camile de Barros Lopes, Ândrea Kely Campos Ribeiro dos Santos, Ana Paula Negreiros Nunes Alves, Fabrício Bitu Sousa, Paulo Goberlânio de Barros Silva, Clarissa Pessoa Fernandes Forte |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male medicine.medical_specialty Doenças Cardiovasculares Statistics as Topic Dental Plaque Heart Valve Diseases Dentistry Observational Study Dental plaque Real-Time Polymerase Chain Reaction Gastroenterology Prevotella intermedia Streptococcus mutans Gingivitis stomatognathic system Internal medicine Medicine Humans Heart valve Saliva Periodontal Diseases Aged Periodontitis Mouth biology business.industry Calculus (dental) Treponema denticola General Medicine Middle Aged biology.organism_classification medicine.disease Heart Valves stomatognathic diseases medicine.anatomical_structure Bactérias Female medicine.symptom business Porphyromonas gingivalis Research Article |
| Zdroj: | Medicine Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC |
| ISSN: | 1536-5964 |
| Popis: | Structural deficiencies and functional abnormalities of heart valves represent an important cause of cardiovascular morbidity and mortality, and a number of diseases, such as aortic stenosis, have been recently associated with infectious agents. This study aimed to analyze oral bacteria in dental plaque, saliva, and cardiac valves of patients with cardiovascular disease. Samples of supragingival plaque, subgingival plaque, saliva, and cardiac valve tissue were collected from 42 patients with heart valve disease. Molecular analysis of Streptococcus mutans, Prevotella intermedia, Porphyromonas gingivalis, and Treponema denticola was performed through real-time PCR. The micro-organism most frequently detected in heart valve samples was the S. mutans (89.3%), followed by P. intermedia (19.1%), P. gingivalis (4.2%), and T. denticola (2.1%). The mean decayed, missing, filled teeth (DMFT) was 26.4 ± 6.9 (mean ± SD), and according to the highest score of periodontal disease observed for each patient, periodontal pockets > 4 mm and dental calculus were detected in 43.4% and 34.7% of patients, respectively. In conclusion, oral bacteria, especially S. mutans, were found in the cardiac valve samples of patients with a high rate of caries and gingivitis/periodontitis. |
| Databáze: | OpenAIRE |
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