Fabrication of nano-hydroxyapatite/collagen/osteonectin composites for bone graft applications
| Autor: | Seeram Ramakrishna, Casey K. Chan, Susan Liao, Michelle Ngiam |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Materials science
Bone Regeneration Scanning electron microscope Biomedical Engineering Bioengineering Bone and Bones law.invention Biomaterials Microscopy Electron Transmission X-Ray Diffraction law Biomimetics Spectroscopy Fourier Transform Infrared medicine Humans Nanotechnology Osteonectin chemistry.chemical_classification biology Tissue Engineering Scleroprotein Osteoblast Anatomy musculoskeletal system Nanostructures medicine.anatomical_structure Durapatite chemistry Transmission electron microscopy Nanofiber Bone Substitutes biology.protein Microscopy Electron Scanning Collagen Electron microscope Type I collagen Biomedical engineering |
| Zdroj: | Biomedical materials (Bristol, England). 4(2) |
| ISSN: | 1748-605X |
| Popis: | Mineralized type I collagen (collagen I) nanofibers and their nanofibril bundles make up the microstructure of natural bone tissue, which range from nanometers to micrometers. However, attempts to achieve this hierarchically assembled structure in vitro have been unsuccessful. In this study, we added osteonectin into the collagen I solution, either at a high or low weight ratio (osteonectin: collagen I = 1:30 or 1:90) before co-precipitation. Results indicated that spindle-like nano-hydroxyapatite (nano-HA) was deposited on collagen/osteonectin and pure osteonectin (control) groups. Furthermore, transmission electron microscope (TEM) and scanning electron microscope (SEM) results showed that the assembled mineralized fiber bundles were formed randomly at different levels from 50 nm, 250 nm to 1100 nm. However, when we replaced collagen I with collagen II, osteonectin addition did not induce the formation of mineralized fiber bundles. The participation of osteonectin in the assembly of the mineralized fibers could provide new insights into the novel mineralization function of osteonectin for bone development in vivo and advancing new biomimetic methods for bone graft applications. |
| Databáze: | OpenAIRE |
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