Role of B Cells in Mucosal Vaccine–Induced Protective CD8+ T Cell Immunity against Pulmonary Tuberculosis
| Autor: | Anna Zganiacz, Amandeep Khera, Zhou Xing, Sam Afkhami, Daniela Damjanovic, Rocky Lai, Joni Hammill, Talveer S. Mandur, Mangalakumari Jeyanathan |
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| Rok vydání: | 2015 |
| Předmět: |
Adoptive cell transfer
T cell Immunology Mice SCID Respiratory Mucosa CD8-Positive T-Lymphocytes Biology Lymphocyte Depletion Mice Antigen Immunity medicine Animals Immunology and Allergy Cytotoxic T cell Tuberculosis Vaccines Immunity Mucosal Tuberculosis Pulmonary B cell Mice Knockout Antigens Bacterial B-Lymphocytes Mice Inbred BALB C Vaccination Mycobacterium tuberculosis Adoptive Transfer medicine.anatomical_structure Mucosal immunology Female Tuberculosis vaccines Acyltransferases |
| Zdroj: | The Journal of Immunology. 195:2900-2907 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Emerging evidence suggests a role of B cells in host defense against primary pulmonary tuberculosis (TB). However, the role of B cells in TB vaccine–induced protective T cell immunity still remains unknown. Using a viral-vectored model TB vaccine and a number of experimental approaches, we have investigated the role of B cells in respiratory mucosal vaccine–induced T cell responses and protection against pulmonary TB. We found that respiratory mucosal vaccination activated Ag-specific B cell responses. Whereas respiratory mucosal vaccination elicited Ag-specific T cell responses in the airway and lung interstitium of genetic B cell–deficient (Jh−/− knockout [KO]) mice, the levels of airway T cell responses were lower than in wild-type hosts, which were associated with suboptimal protection against pulmonary Mycobacterium tuberculosis challenge. However, mucosal vaccination induced T cell responses in the airway and lung interstitium and protection in B cell–depleted wild-type mice to a similar extent as in B cell–competent hosts. Furthermore, by using an adoptive cell transfer approach, reconstitution of B cells in vaccinated Jh−/− KO mice did not enhance anti-TB protection. Moreover, respiratory mucosal vaccine–activated T cells alone were able to enhance anti-TB protection in SCID mice, and the transfer of vaccine-primed B cells alongside T cells did not further enhance such protection. Alternatively, adoptively transferring vaccine-primed T cells from Jh−/− KO mice into SCID mice only provided suboptimal protection. These data together suggest that B cells play a minimal role, and highlight a central role by T cells, in respiratory mucosal vaccine–induced protective immunity against M. tuberculosis. |
| Databáze: | OpenAIRE |
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