Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorus predation
| Autor: | Rob Till, Joe W. Gray, Waldemar Vollmer, Yves V. Brun, Adrien Ducret, Erkin Kuru, R. Elizabeth Sockett, Jacob Biboy, Michael S. VanNieuwenhze, Carey Lambert, Jonathan Rittichier, Adeline Derouaux |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Time Factors 030106 microbiology Immunology Peptidoglycan Applied Microbiology and Biotechnology Microbiology Article Bdellovibrio Bacterial genetics Cell wall 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Cell Wall Gram-Negative Bacteria Escherichia coli Genetics Amino Acids Sequence Deletion chemistry.chemical_classification biology Amino Acids Diamino Cell Biology Bdellovibrio bacteriovorus biology.organism_classification Amino acid chemistry Genes Bacterial Peptidyl Transferases Biophysics Intracellular Bacteria |
| Zdroj: | Nature microbiology |
| ISSN: | 2058-5276 |
| Popis: | Modification of essential bacterial peptidoglycan (PG) containing cell walls can lead to antibiotic resistance, for example β-lactam resistance by L,D-transpeptidase activities. Predatory Bdellovibrio bacteriovorus are naturally antibacterial and combat infections by traversing, modifying and finally destroying walls of Gram-negative prey bacteria, modifying their own PG as they grow inside prey. Historically, these multi-enzymatic processes on two similar PG walls have proved challenging to elucidate. Here, with a PG labelling approach utilizing timed pulses of multiple fluorescent D-amino acids (FDAAs), we illuminate dynamic changes that predator and prey walls go through during the different phases of bacteria:bacteria invasion. We show formation of a reinforced circular port-hole in the prey wall; L,D-transpeptidaseBd mediated D-amino acid modifications strengthening prey PG during Bdellovibrio invasion and a zonal mode of predator-elongation. This process is followed by unconventional, multi-point and synchronous septation of the intracellular Bdellovibrio, accommodating odd- and even-numbered progeny formation by non-binary division. |
| Databáze: | OpenAIRE |
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