Down-regulation of EMP1 is associated with epithelial hyperplasia and metaplasia in nasal polyps
| Autor: | De Yun Wang, Chun Wei Li, Tian Ying Li, Li Zhao, Xue Min Yu, Zhi Bin Lin, Jing Liu, Li Shi, Xin Liang Pan, Yingying Li |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Histology Anti-Inflammatory Agents Down-Regulation Mucous membrane of nose Receptors Cell Surface Biology Epithelial membrane protein-1 Epithelium Pathology and Forensic Medicine Basal (phylogenetics) Nasal Polyps Metaplasia medicine Humans Nasal polyps Pregnadienediols Hyperplasia General Medicine Middle Aged medicine.disease Neoplasm Proteins Nasal Mucosa medicine.anatomical_structure Immunohistochemistry Female medicine.symptom Mometasone Furoate |
| Zdroj: | Histopathology. 63(5) |
| ISSN: | 1365-2559 |
| Popis: | Aims The aim of this study was to assess protein and mRNA expression of epithelial membrane protein 1 (EMP1) in the nasal mucosa of patients with nasal polyps (NP), and to determine what changes occur in response to glucocorticosteroid (GC) treatment. Methods and results NP tissue was obtained from 55 patients, 18 of whom were treated with nasal GCs (i.e. these 18 patients had NP biopsies taken before and after treatment). Biopsies of inferior turbinate mucosa from 30 healthy subjects were used as controls. Quantitative PCR and immunohistochemistry were performed to determine the expression levels of EMP1. EMP1 mRNA expression was significantly lower (2.77-fold) in tissues from NP patients before GC treatment when compared to controls, but was increased in these patients after GC treatment. EMP1 staining in nasal epithelium co-localized with both basal (p63+) and differentiated (CK18+) epithelial cells. Their immunoreactivity was significantly greater in controls than NP patients. EMP1 mRNA levels were lower in the epithelium with severe hyperplasia (1.79-fold) or with metaplasia (1.85-fold) as compared to those with mild to moderate hyperplasia or non-metaplastic epithelium, respectively. Positive correlations between EMP1 and other epithelial cell-related gene (e.g. JUN, PTGS2, AREG etc.) mRNAs were observed. Conclusions EMP1 could be a biomarker for aberrant epithelial remodelling and metaplasia in chronic inflammatory upper airway mucosa (e.g. NP). |
| Databáze: | OpenAIRE |
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