Chemical Regeneration of Wound Defects: Relevance to the Canine Palatal Mucosa and Cell Cycle Up-Regulation in Human Gingival Fibroblasts
| Autor: | Richard Leesungbok, Suk-Won Lee, Kyung-Ho Lee, Heithem Ben Amara, Ki-Tae Koo, Sang Cheon Lee, Min Ah Chung |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_treatment
0206 medical engineering Biomedical Engineering Gingiva Medicine (miscellaneous) 02 engineering and technology Andrology 03 medical and health sciences Dogs In vivo medicine Animals Humans Regeneration Viability assay Oral mucosa Trichloroacetic Acid 030304 developmental biology 0303 health sciences Chemistry Cell growth Palate Regeneration (biology) Growth factor Mouth Mucosa Cell Cycle Checkpoints Cell cycle Fibroblasts 020601 biomedical engineering Up-Regulation medicine.anatomical_structure Intercellular Signaling Peptides and Proteins Original Article Wound healing Signal Transduction |
| Zdroj: | Tissue Eng Regen Med |
| ISSN: | 2212-5469 |
| Popis: | BACKGROUND: Trichloroacetic acid (TCA) is an agent widely applied in dermatology for skin regeneration. To test whether TCA can offer an advantage for the regeneration of oral soft tissue defects, the cellular events following TCA application were explored in vitro and its influence on the oral soft tissue wound healing was evaluated in a canine palate model. METHODS: The cytotoxicity and growth factor gene expression in human gingival fibroblasts were tested in vitro following the application of TCA at four concentrations (0.005%, 0.05%, 0.5% and 1%) with different time intervals (0, 3, 9 and 21 h). One concentration of TCA was selected to screen the genes differentially expressed using DNA microarray and the associated pathways were explored. TCA was injected in open wound defects of the palatal mucosa from beagle dogs (n = 3) to monitor their healing and regeneration up to day 16-post-administration. RESULTS: While the 0.5–1% concentration induced the cytoxicity, a significantly higher expression of growth factor genes was observed after 3 and 9 h following the 0.5% TCA application in comparison to other groups. DNA microarray analysis in 0.5% TCA group showed 417 genes with a significant 1.5-fold differential expression, involving pathways of cell cycle, FoxO signaling, p53 signaling, ubiquitin mediated proteolysis and cAMP signaling. In vivo results showed a faster reepithelialization of TCA-treated wounds as compared to spontaneous healing. CONCLUSION: TCA promoted the healing and regeneration of oral soft tissue wound defects by up-regulating the cell cycle progression, cell growth, and cell viability, particularly at a concentration of 0.5%. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13770-019-00227-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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