Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
| Autor: | Myung Chul Choi, Siyoung Q. Choi, Dae-Woong Jeong, KyuHan Kim |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Microscope
Materials science drop coalescence General Chemical Engineering fluid-fluid interfaces Bioengineering General Biochemistry Genetics and Molecular Biology law.invention Surface-Active Agents inter-diffusion law phospholipid monolayer Microscopy Monolayer Fluorescence microscope Phospholipids Coalescence (physics) Photobleaching General Immunology and Microbiology General Neuroscience diffusion Fluorescence recovery after photobleaching Water Fluorescence FRAM Microscopy Fluorescence Chemical physics Issue 104 FRAP Fluorescence Recovery After Photobleaching |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| Popis: | We introduce a new method to measure the lateral diffusivity of a surfactant monolayer at the fluid-fluid interface, called fluorescence recovery after merging (FRAM). FRAM adopts the same principles as the fluorescence recovery after photobleaching (FRAP) technique, especially for measuring fluorescence recovery after bleaching a specific area, but FRAM uses a drop coalescence instead of photobleaching dye molecules to induce a chemical potential gradient of dye molecules. Our technique has several advantages over FRAP: it only requires a fluorescence microscope rather than a confocal microscope equipped with high power lasers; it is essentially free from the selection of fluorescence dyes; and it has far more freedom to define the measured diffusion area. Furthermore, FRAM potentially provides a route for studying the mixing or inter-diffusion of two different surfactants, when the monolayers at a surface of droplet and at a flat air/water interface are prepared with different species, independently. |
| Databáze: | OpenAIRE |
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