The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants
| Autor: | Shigeo Nakamura, Yumi Naito, Chi Ni Hsia, Yoshitaka Sano, Yuko Ohashi, Ichiro Mitsuhara, Naomi Shirasawa-Seo, Yoko Gotoh, Shigemi Seo, Shunichi Kosugi, Taka Murakami |
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| Rok vydání: | 2005 |
| Předmět: |
Nicotiana tabacum
Molecular Sequence Data GUS reporter system Plant Science Virus Viral Proteins Soybean chlorotic mottle virus Tobacco Gene expression Cloning Molecular Promoter Regions Genetic Genetics Regulation of gene expression Base Sequence biology Nanovirus fungi Gene Expression Regulation Developmental food and beverages Oryza General Medicine Plants Genetically Modified biology.organism_classification Genetically modified rice Cauliflower mosaic virus Agronomy and Crop Science |
| Zdroj: | Plant Cell Reports. 24:155-163 |
| ISSN: | 1432-203X 0721-7714 |
| Popis: | The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35S but with a more intense expression in proliferating tissues. The usefulness of PMC8 was confirmed by driving NPTII for selection of kanamycin-resistant tobacco plants with improved transformation efficiency. PMC8 was also effective in transgenic rice plants. Thus, PMC8 is useful as an alternative to P35S in both dicotyledonous and monocotyledonous plants, especially for gene expression in proliferating tissues. |
| Databáze: | OpenAIRE |
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