Anaplasma phagocytophilum Antibodies in Humans, Japan, 2010–2011
| Autor: | Dongxing Wu, Asaka Ikegaya, Yuko Yoshikawa, Daisuke Takechi, Katsumi Aso, Kazuhito Saitoh, Fumihiko Kawamori, Shuji Ando, Norio Ohashi, Takuya Watanabe, Daisuke Shichi, Gaowa, Yoichi Murakami |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Letter Human granulocytic anaplasmosis P44 immunodominant protein Epidemiology animal diseases vector-borne infections lcsh:Medicine Biology p44/msp2 lcsh:Infectious and parasitic diseases Serology Antigen Japan parasitic diseases medicine lcsh:RC109-216 anaplasmosis Letters to the Editor bacteria serodiagnosis lcsh:R bacterial infections and mycoses medicine.disease biology.organism_classification Virology Anaplasma phagocytophilum Infectious Diseases Cell culture biology.protein Anaplasmosis Antibody Bacterial outer membrane |
| Zdroj: | Emerging Infectious Diseases Emerging Infectious Diseases, Vol 20, Iss 3, Pp 508-509 (2014) |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | To the Editor: Human granulocytic anaplasmosis (HGA) is an emerging tick-borne infectious disease caused by Anaplasma phagocytophilum, an obligatory intracellular bacterium (1). Recently, 2 cases of HGA were identified by a retrospective study in Japan (2). For serodiagnosis of HGA, A. phagocytophilum propagated in HL60 cells is usually used as an antigen, especially by indirect immunofluorescent assay (IFA) (3). However, the serum from these 2 patients in Japan reacted with antigens of A. phagocytophilum cultured in THP-1 cells rather than in HL60 cells in IFA (2). In A. phagocytophilum, a p44/msp2 multigene family encoding multiple 44-kDa immunodominant major outer membrane protein species (so-called P44) exists on the genome, and these multigenes are similar, but not identical, to each other, and the bacterium generates antigenic variations because of gene conversion (4). The previous studies showed that A. phagocytophilum expresses predominantly 2 species of p44/msp2 transcripts in THP-1 cells, but it produces the variation of P44 protein species in HL60 cells (2,5). This finding strongly suggested that A. phagocytophilum grown in THP-1 cells differs serologically from that in HL60 cells. Our serologic analysis found 4 recent cases of HGA in Japan by using infected THP-1 and HL60 cells as antigens, and some P44 immunoreactive protein species of A. phagocytophilum that were associated with the respective cell line cultures, binding to antibodies from the 4 patients’ serum, also were identified. |
| Databáze: | OpenAIRE |
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