Apoptotic Cells Provide an Unexpected Source of Wnt3 Signaling to Drive Hydra Head Regeneration
| Autor: | Brigitte Galliot, Kevin Dobretz, Luiza Ghila, Jean-Claude Martinou, Wanda Christa Buzgariu, Christoph Ruediger Bauer, Simona Chera, Yvan Wenger |
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| Rok vydání: | 2009 |
| Předmět: |
Regeneration/physiology
Amino Acid Chloromethyl Ketones/metabolism Hydra Active Transport Cell Nucleus Morphallaxis Context (language use) Apoptosis DEVBIO Biology General Biochemistry Genetics and Molecular Biology Interstitial cell Amino Acid Chloromethyl Ketones S Phase Wnt3 Protein ddc:590 ddc:570 In Situ Nick-End Labeling Regeneration Animals Active Transport Cell Nucleus/physiology Molecular Biology beta Catenin Cell Proliferation Cell growth Signal Transduction/physiology Regeneration (biology) Apoptosis/physiology Cell Biology Anatomy S Phase/physiology Beta Catenin/genetics/metabolism Cell biology Wnt Proteins Imaginal disc Lernaean Hydra Hydra/anatomy & histology/physiology RNA Interference Stem cell Wnt Proteins/genetics/metabolism Signal Transduction Developmental Biology |
| Zdroj: | Developmental Cell, Vol. 17, No 2 (2009) pp. 279-89 |
| ISSN: | 1534-5807 1878-1551 |
| DOI: | 10.1016/j.devcel.2009.07.014 |
| Popis: | SummaryDecapitated Hydra regenerate their heads via morphallaxis, i.e., without significant contributions made by cell proliferation or interstitial stem cells. Indeed, Hydra depleted of interstitial stem cells regenerate robustly, and Wnt3 from epithelial cells triggers head regeneration. However, we find a different mechanism controlling regeneration after midgastric bisection in animals equipped with both epithelial and interstitial cell lineages. In this context, we see rapid induction of apoptosis and Wnt3 secretion among interstitial cells at the head- (but not foot-) regenerating site. Apoptosis is both necessary and sufficient to induce Wnt3 production and head regeneration, even at ectopic sites. Further, we identify a zone of proliferation beneath the apoptotic zone, reminiscent of proliferative blastemas in regenerating limbs and of compensatory proliferation induced by dying cells in Drosophila imaginal discs. We propose that different types of injuries induce distinct cellular modes of Hydra head regeneration, which nonetheless converge on a central effector, Wnt3. |
| Databáze: | OpenAIRE |
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