A Role for WNT Proteins in Induction of Dermomyotome
Autor: | Marc Tessier-Lavigne, Chen-Ming Fan, Catherine S. Lee |
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Rok vydání: | 1997 |
Předmět: |
Polymerase Chain Reaction
Mesoderm Mice somite Wnt4 Protein Sonic hedgehog In Situ Hybridization Embryonic Induction patterning Anatomy Surface ectoderm Recombinant Proteins Cell biology medicine.anatomical_structure COS Cells embryonic structures Signal Transduction animal structures Notochord Dermomyotome Wnt1 Protein Biology Transfection Cell Line Wnt3 Protein Organ Culture Techniques Proto-Oncogene Proteins Wnt3A Protein Ectoderm Paraxial mesoderm medicine Animals Hedgehog Proteins Molecular Biology Floor plate Body Patterning Neural tube Proteins Cell Biology Zebrafish Proteins Rats Wnt Proteins Somite dermomyotome Protein Biosynthesis biology.protein Trans-Activators Wnt SHH Developmental Biology |
Zdroj: | Developmental Biology. 191(1):160-165 |
ISSN: | 0012-1606 |
DOI: | 10.1006/dbio.1997.8713 |
Popis: | Dorsoventral patterning of somites into sclerotome and dermomyotome involves antagonistic actions of ventralizing and dorsalizing signals originating from tissues surrounding the somites. The notochord and the floor plate of the neural tube provide a ventralizing signal(s) directing sclerotome development, whereas the surface ectoderm and dorsal neural tube provide a dorsalizing signal(s) directing dermomyotome development. Evidence has been provided that Sonic Hedgehog mediates the ventralizing effects of notochord and floor plate, but the dorsalizing signal(s) that patterns the dermomyotome has not been identified. The documented expression of Wnt1 and Wnt3a in the dorsal neural tube and of Wnt4 and Wnt6 in the surface ectoderm at the time of dermomyotome specification prompted us to investigate the involvement of WNT proteins in patterning the dermomyotome. Here we show that tissue culture cells expressing these WNT family members can maintain and induce dermomyotome marker expression in presomitic mesoderm explants, supporting the hypothesis that WNT proteins mediate the dorsalizing effects of the surface ectoderm and dorsal neural tube on somites. |
Databáze: | OpenAIRE |
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