Inhibition of Human Immunodeficiency Virus-1 Proliferation by Liposome-Encapsulated Sense DNA to the 5′TATSplice Acceptor Site
| Autor: | Thomas G. Gabrysiak, Jörg Ruppert, Sean M. Sullivan, Michael J. Scolaro, W. John Martin, J. Hinrich Peters, Gerry Cox, Leroy Richer, Robert K. Gieseler, Susanne Lenzner |
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| Rok vydání: | 1992 |
| Předmět: |
RNA Splicing
Molecular Sequence Data DNA Recombinant Biology Virus Replication Antiviral Agents DNA Antisense Virus chemistry.chemical_compound Sense (molecular biology) Leukocytes Genetics Humans A-DNA splice Drug Carriers Liposome Base Sequence Oligonucleotides Antisense Acceptor Molecular biology Endocytosis chemistry Genes tat Liposomes HIV-1 Intracellular DNA |
| Zdroj: | Antisense Research and Development. 2:187-197 |
| ISSN: | 1050-5261 |
| DOI: | 10.1089/ard.1992.2.187 |
| Popis: | A liposome formulation containing a distearoylphosphatidylethanolamine analog was developed that was endocytosed by both lymphocytes and monocytes. This formulation was used to encapsulate sense and antisense 20-mer oligodeoxynucleotides to the 5' tat splice acceptor site of human immunodeficiency virus type 1. At a DNA concentration of 140 nM, the liposome-encapsulated sense DNA inhibited p24 production by as much as 84% in human peripheral blood leukocytes infected with "wild-type" virus. This treatment also reduced the number of peripheral blood leukocytes producing intracellular viral antigen by 71%. Of interest, no reduction in either parameter was observed for the antisense-containing liposomes. The results demonstrate the promise of a new liposomal delivery vehicle to inhibit human immunodeficiency virus replication by an entrapped oligodeoxynucleotide. |
| Databáze: | OpenAIRE |
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