Stochastic sensing of Angiotensin II with lysenin channels
| Autor: | Christopher A. Thomas, Daniel Fologea, Sheenah Bryant, Juliette K. Tinker, Devon Richtsmeier, Xinzhu Pu, Nisha Shrestha |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Analyte Science Lipid Bilayers Synthetic membrane Peptide Biosensing Techniques Biology Article 03 medical and health sciences Animals Oligochaeta Toxins Biological chemistry.chemical_classification Chitosan Multidisciplinary Angiotensin II Biological Transport Sphingomyelins Nanopore 030104 developmental biology Membrane chemistry Biochemistry Medicine Sphingomyelin Ion Channel Gating Macromolecule |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The ability of pore-forming proteins to interact with various analytes has found vast applicability in single molecule sensing and characterization. In spite of their abundance in organisms from all kingdoms of life, only a few pore-forming proteins have been successfully reconstituted in artificial membrane systems for sensing purposes. Lysenin, a pore-forming toxin extracted from the earthworm E. fetida, inserts large conductance nanopores in lipid membranes containing sphingomyelin. Here we show that single lysenin channels may function as stochastic nanosensors by allowing the short cationic peptide angiotensin II to be electrophoretically driven through the conducting pathway. Long-term translocation experiments performed using large populations of lysenin channels allowed unequivocal identification of the unmodified analyte by Liquid Chromatography-Mass Spectrometry. However, application of reverse voltages or irreversible blockage of the macroscopic conductance of lysenin channels by chitosan addition prevented analyte translocation. This investigation demonstrates that lysenin channels have the potential to function as nano-sensing devices capable of single peptide molecule identification and characterization, which may be further extended to other macromolecular analytes. |
| Databáze: | OpenAIRE |
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