A single-nucleotide polymorphism in tumor necrosis factor-α (−308 G/A) as a biomarker in chronic pancreatitis
| Autor: | B. Prabhakar, M. Ramanna, M. Uma Devi, Pratibha Nallari, Ananthapur Venkateshwari, K. Sri Manjari, Akka Jyothy, P. Shravan Kumar |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Genotype Single-nucleotide polymorphism Disease Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Gastroenterology Young Adult Pancreatitis Chronic Internal medicine Genetics medicine Humans Genetic Predisposition to Disease Prospective Studies Allele Promoter Regions Genetic Genotyping Aged DNA Primers Tumor Necrosis Factor-alpha General Medicine Middle Aged medicine.disease Endocrinology Case-Control Studies Etiology Pancreatitis Female Tumor necrosis factor alpha Biomarkers |
| Zdroj: | Gene. 539:186-189 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2014.02.014 |
| Popis: | Chronic pancreatitis is a gradual, long-term inflammation of the pancreas that results in alteration of its normal structure and function. The study aims to investigate the role of -308 (G/A) polymorphism of TNF-α gene in chronic pancreatitis.A total of 200 subjects were included in this case-control study. A total of 100 in patients admitted in the Gastroenterology Unit of Gandhi Hospital and Osmania General Hospital, Hyderabad were included in the present study. An equal number of healthy control subjects were randomly selected for the study. The genotyping of TNF-α gene was carried out by tetra-primer ARMS PCR followed by gel electrophoresis. The TNF-α levels were assayed by enzyme-linked immunosorbent assay.A significant variation with respect to the genotypic and allelic distribution in the disease group when compared to control subjects [OR=2.001 (1.33-3.005), p0.0001**] was observed. Subjects homozygous for the A allele had higher TNF-α levels compared to G allele.The present study revealed a significant association of the TNF-α gene promoter polymorphism with chronic pancreatitis. Thus, TNF-α genotype can be considered as one of the biological markers in the etiology of chronic pancreatitis. |
| Databáze: | OpenAIRE |
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