Immunoactivation of lectin-conjugated praecoxin A on IL-6, IL-12 expression
| Autor: | Do Ik Lee, Seong Soo Joo, Jeong Hwan Park, Jae Kwon Chang, Hee Chul Kang |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Wheat Germ Agglutinins Mice Immune system Drug Discovery Macrophage Animals RNA Messenger Interleukin 6 Cells Cultured Messenger RNA Mice Inbred ICR biology Interleukin-6 Organic Chemistry Lectin Macrophage Activation Molecular biology Interleukin-12 In vitro Wheat germ agglutinin biology.protein Interleukin 12 Macrophages Peritoneal Molecular Medicine Plant Lectins Tannins |
| Zdroj: | Archives of pharmacal research. 25(6) |
| ISSN: | 0253-6269 |
| Popis: | Lectin-conjugated praecoxin A is a compound, which is combined Wheat Germ Agglutinin (WGA) Lectin with praecoxin A and also known to have an anti-tumor activity. In our lab, in order to investigate its immune reaction other than the anti-tumor activity ever known, we examined cytokines such as IL-6 and IL-12 through their mRNA expressions, which are generally secreted by macrophage both in vivo and in vitro. To analyze, we used RT-PCR for total RNAs of macrophages. As a result, we obtained that both in vitro and in vivo, lectin-conjugated praecoxin A showed an interesting increase on IL-6 and IL-12 even though it may be little hard to say the conjugated form is absolutely more effective than that of lectin or praecoxin A alone for immune response activities. Those results suggest that the conjugated form may give an additional opportunity in a future therapeutic use over its immuno activation properties. |
| Databáze: | OpenAIRE |
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