Bacteroides fragilis prevents Salmonella Heidelberg translocation in co-culture model mimicking intestinal epithelium
| Autor: | T. Vernay, François Gaboriau, Zohreh Tamanai-Shacoori, Anne Jolivet-Gougeon, Olivier Loréal, Isabelle Cannie, A Burel, S David-Le Gall, Latifa Bousarghin |
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| Přispěvatelé: | Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), H2P2 - Histo Pathologie Hight Precision (H2P2), Université de Rennes (UR)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Université de Rennes 1 (UR1) |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Serotype goblet cells [SDV]Life Sciences [q-bio] 030106 microbiology Chromosomal translocation Microbiology law.invention 03 medical and health sciences Probiotic law Bacteroides M cells Microfold cell biology Chemistry biology.organism_classification Intestinal epithelium 030104 developmental biology enterocytes gut permeability Bacteroides fragilis Bacteria |
| Zdroj: | Beneficial Microbes Beneficial Microbes, 2020, 11 (4), pp.391-401. ⟨10.3920/BM2020.0004⟩ Beneficial Microbes, Wageningen Academic Publishers 2020, 11 (4), pp.391-401. ⟨10.3920/BM2020.0004⟩ |
| ISSN: | 1876-2891 1876-2883 |
| DOI: | 10.3920/bm2020.0004 |
| Popis: | International audience; Salmonella Heidelberg is one of the most common serovar causing foodborne illnesses. To limit the development of digestive bacterial infection, food supplements containing probiotic bacteria can be proposed. Commensal non-toxigenic Bacteroides fragilis has recently been suggested as a next-generation probiotic candidate. By using an original triple co-culture model including Caco-2 cells (representing human enterocytes), HT29-MTX (representing mucus-secreting goblet cells), and M cells differentiated from Caco-2 by addition of Raji B lymphocytes, bacterial translocation was evaluated. The data showed that S. Heidelberg could translocate in the triple co-culture model with high efficiency, whereas for B. fragilis a weak translocation was obtained. When cells were exposed to both bacteria, S. Heidelberg translocation was inhibited. The cell-free supernatant of B. fragilis also inhibited S. Heidelberg translocation without impacting epithelial barrier integrity. This supernatant did not affect the growth of S. Heidelberg. The non-toxigenic B. fragilis confers health benefits to the host by reducting bacterial translocation. These results suggested that the multicellular model provides an efficient in vitro model to evaluate the translocation of pathogens and to screen for probiotics that have a potential inhibitory effect on this translocation. |
| Databáze: | OpenAIRE |
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