Exploring the brain tissue proteome of TgCRND8 Alzheimer's Disease model mice under B vitamin deficient diet induced hyperhomocysteinemia by LC-MS top-down platform
| Autor: | Federica Vincenzoni, Massimo Castagnola, Andrea Urbani, Andrea Fuso, Claudia Desiderio, Sigfrido Scarpa, Claudia Martelli, Rosaria A. Cavallaro, Daniela Delfino, Diana Valeria Rossetti, Ilaria Inserra |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Proteome Clinical Biochemistry Proteomics Alzheimer's Disease 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry Mice 0302 clinical medicine Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA biology Chemistry Brain General Medicine Vitamin B Complex Female medicine.medical_specialty Hyperhomocysteinemia alzheimer Brain Acid Soluble Protein 1 Protein subunit Mice Transgenic Nerve Tissue Proteins 03 medical and health sciences Alzheimer Disease Internal medicine medicine Animals Humans Brain Chemistry Chromatography Amyloid beta-Peptides 010401 analytical chemistry Wild type Cell Biology TgCRND8 mice medicine.disease Metallothionein 3 0104 chemical sciences B vitamins Cytoskeletal Proteins Disease Models Animal Endocrinology biology.protein Calmodulin-Binding Proteins Protein G Chromatography Liquid |
| Zdroj: | Journal of chromatography. B 1124 (2019): 165–172. doi:10.1016/j.jchromb.2019.06.005 info:cnr-pdr/source/autori:Delfino, Daniela; Rossetti, Diana Valeria; Martelli, Claudia; Inserra, Ilaria; Vincenzoni, Federica; Castagnola, Massimo; Urbani, Andrea; Scarpa, Sigfrido; Fuso, Andrea; Cavallaro, Rosaria A.; Desiderio, Claudia/titolo:Exploring the brain tissue proteome of TgCRND8 Alzheimer's Disease model mice under B vitamin deficient diet induced hyperhomocysteinemia by LC-MS top-down platform/doi:10.1016%2Fj.jchromb.2019.06.005/rivista:Journal of chromatography. B (Print)/anno:2019/pagina_da:165/pagina_a:172/intervallo_pagine:165–172/volume:1124 |
| DOI: | 10.1016/j.jchromb.2019.06.005 |
| Popis: | The multifactorial nature of Late Onset Alzheimer's Disease (LOAD), the AD form of major relevance on epidemiological and social aspects, has driven the original investigation by LC-MS and top-down proteomics approach of the protein repertoire of the brain tissue of TgCRND8 model mice fed with a diet deficient in B vitamins. The analysis of the acid-soluble fraction of brain tissue homogenates identified a list of proteins and peptides, proteoforms and PTMs. In order to disclose possible modulations, their relative quantification in wild type and AD model mice under both B vitamin deficient and control diets was performed. The levels of metallothionein III, guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 and brain acid soluble protein 1 showed statistically significant alterations depending on genotype, diet or both effects, respectively. Particularly, metallothionein III exhibited increased levels in TgCRND8 mice under B vitamin deficient diet with respect to wild type mice under both diets. Brain acid soluble protein 1 showed the opposite, revealing decreased levels in all diet groups of AD model mice with respect to wild type mice in control diet. Lower levels of brain acid soluble protein 1 were also observed in wild type mice under deficiency of B vitamins. These results, besides contributing to increase the knowledge of AD at molecular level, give new suggestions for deeply investigating metallothionein III and brain acid soluble protein 1 in AD. |
| Databáze: | OpenAIRE |
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