Control of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)
| Autor: | Antonio Olavo Cardoso Jorge, Jonatas Rafael de Oliveira, Rosilene Fernandes da Rocha, Luciane Dias de Oliveira, Felipe Eduardo de Oliveira, Rosilene Batista de Aguiar Almeida, Polyana das Graças Figueiredo Vilela |
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| Rok vydání: | 2013 |
| Předmět: |
Staphylococcus aureus
Interleukin-1beta Acrylic Resins Cell Culture Techniques Colony Count Microbial Candida glabrata medicine.disease_cause Microbiology Streptococcus mutans Anti-Infective Agents Candida albicans medicine Staphylococcus epidermidis MTT assay Candida tropicalis General Dentistry biology Chemistry Plant Extracts Tumor Necrosis Factor-alpha Macrophages Broth microdilution Cell Biology General Medicine biology.organism_classification Antimicrobial Plankton Corpus albicans Arctium Otorhinolaryngology Biofilms Arctium lappa |
| Zdroj: | Archives of oral biology. 59(8) |
| ISSN: | 1879-1506 |
| Popis: | Objectives To evaluate the antimicrobial activity of Arctium lappa L. extract on Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Candida albicans, C. tropicalis and C. glabrata. In addition, the cytotoxicity of this extract was analyzed on macrophages (RAW 264.7). Design By broth microdilution method, different concentrations of the extract (250–0.4 mg/mL) were used in order to determine the minimum microbicidal concentration (MMC) in planktonic cultures and the most effective concentration was used on biofilms on discs made of acrylic resin. The cytotoxicity A. lappa L. extract MMC was evaluated on RAW 264.7 by MTT assay and the quantification of IL-1β and TNF-α by ELISA. Results The most effective concentration was 250 mg/mL and also promoted significant reduction (log10) in the biofilms of S. aureus (0.438 ± 0.269), S. epidermidis (0.377 ± 0.298), S. mutans (0.244 ± 0.161) and C. albicans (0.746 ± 0.209). Cell viability was similar to 100%. The production of IL-1β was similar to the control group (p > 0.05) and there was inhibition of TNF-α (p Conclusions A. lappa L. extract was microbicidal for all the evaluated strains in planktonic cultures, microbiostatic for biofilms and not cytotoxic to the macrophages. |
| Databáze: | OpenAIRE |
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