Gene expression profiling of Spodoptera frugiperda hemocytes and fat body using cDNA microarray reveals polydnavirus-associated variations in lepidopteran host genes transcript levels

Autor: Jousset Fx, Anne-Nathalie Volkoff, Galibert L, Frédérique Hilliou, René Feyereisen, Philippe Fournier, Pierre Delobel, Sofer L, Marc Ravallec, Barat-Houari M, Emeline Deleury, Janick Rocher
Přispěvatelé: Biologie Intégrative et Virologie des Insectes [Univ. de Montpellier II] (BIVI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Réponse des Organismes aux Stress Environnementaux (ROSE), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Hemocytes
Fat Body
TRANSCRIPTIONAL VARIATIONS
Autoantigens
SPODOPTERA FRUGIPERDA
cDNA MICROARRAY
GENE EXPRESSION
POLYDNAVIRUS
IMMUNE RESPONSE
LEPIDOPTERA
RELATION HOTE PARASITOIDE
Oligonucleotide Array Sequence Analysis
Genetics
Receptors
Scavenger
0303 health sciences
Enzyme Precursors
biology
Polydnavirus
030302 biochemistry & molecular biology
Prophenoloxidase
Insect Proteins
Female
DNA microarray
Catechol Oxidase
Biotechnology
Research Article
expression génique
lcsh:QH426-470
Galectins
lcsh:Biotechnology
réponse immunitaire
Genes
MHC Class II
Down-Regulation
Spodoptera
03 medical and health sciences
Complementary DNA
lcsh:TP248.13-248.65
Animals
Lectins
C-Type
RNA
Messenger
Selection
Genetic
Gene
030304 developmental biology
[SDV.GEN]Life Sciences [q-bio]/Genetics
Innate immune system
Gene Expression Profiling
fungi
Genetic Variation
biology.organism_classification
Immunity
Innate
Gene expression profiling
Protein Subunits
lcsh:Genetics
Polydnaviridae
Calreticulin
Zdroj: BMC Genomics, Vol 7, Iss 1, p 160 (2006)
BMC Genomics
BMC Genomics, BioMed Central, 2006, 7 (160), n. p. ⟨10.1186/1471-2164-7-160⟩
Europe PubMed Central
BMC Genomics 160 (7), n. p.. (2006)
ISSN: 1471-2164
DOI: 10.1186/1471-2164-7-160⟩
Popis: Background Genomic approaches provide unique opportunities to study interactions of insects with their pathogens. We developed a cDNA microarray to analyze the gene transcription profile of the lepidopteran pest Spodoptera frugiperda in response to injection of the polydnavirus HdIV associated with the ichneumonid wasp Hyposoter didymator. Polydnaviruses are associated with parasitic ichneumonoid wasps and are required for their development within the lepidopteran host, in which they act as potent immunosuppressive pathogens. In this study, we analyzed transcriptional variations in the two main effectors of the insect immune response, the hemocytes and the fat body, after injection of filter-purified HdIV. Results Results show that 24 hours post-injection, about 4% of the 1750 arrayed host genes display changes in their transcript levels with a large proportion (76%) showing a decrease. As a comparison, in S. frugiperda fat body, after injection of the pathogenic JcDNV densovirus, 8 genes display significant changes in their transcript level. They differ from the 7 affected by HdIV and, as opposed to HdIV injection, are all up-regulated. Interestingly, several of the genes that are modulated by HdIV injection have been shown to be involved in lepidopteran innate immunity. Levels of transcripts related to calreticulin, prophenoloxidase-activating enzyme, immulectin-2 and a novel lepidopteran scavenger receptor are decreased in hemocytes of HdIV-injected caterpillars. This was confirmed by quantitative RT-PCR analysis but not observed after injection of heat-inactivated HdIV. Conversely, an increased level of transcripts was found for a galactose-binding lectin and, surprisingly, for the prophenoloxidase subunits. The results obtained suggest that HdIV injection affects transcript levels of genes encoding different components of the host immune response (non-self recognition, humoral and cellular responses). Conclusion This analysis of the host-polydnavirus interactions by a microarray approach indicates that the presence of HdIV induces, directly or indirectly, variations in transcript levels of specific host genes, changes that could be responsible in part for the alterations observed in the parasitized host physiology. Development of such global approaches will allow a better understanding of the strategies employed by parasites to manipulate their host physiology, and will permit the identification of potential targets of the immunosuppressive polydnaviruses.
Databáze: OpenAIRE
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