Long noncoding RNA TUG1 promotes osteogenic differentiation of human periodontal ligament stem cell through sponging microRNA-222-3p to negatively regulate Smad2/7
| Autor: | Fang Wang, Sun Degang, Qishan Wu, Di Wu, Qian Xu, Bingchang Xin, Lin Yin |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Periodontal ligament stem cells Periodontal Ligament Smad2 Protein Biology Smad7 Protein 03 medical and health sciences 0302 clinical medicine Osteogenesis microRNA medicine Humans Periodontal fiber General Dentistry Periodontitis Gene knockdown Reporter gene Stem Cells Cell Differentiation 030206 dentistry Cell Biology General Medicine medicine.disease Cell biology RUNX2 MicroRNAs 030104 developmental biology Otorhinolaryngology RNA Long Noncoding Stem cell |
| Zdroj: | Archives of Oral Biology. 117:104814 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2020.104814 |
| Popis: | Objective To scrutinize the function of long non-coding RNA TUG1 on the osteogenic differentiation of human periodontal ligament stem cells in periodontitis and its specific mechanism. Methods Human periodontal ligament stem cells were extracted from human periodontium, followed by induction of osteogenic differentiation with osteogenic medium. Knockdown or overexpression of TUG1, microRNA-222-3p or Smad2/7 were performed in human periodontal ligament stem cells to observe their effect on expressions of osteogenic differentiation markers (Runx2, ALP, and OCN), and on calcium nodule formation by Alizarin red staining. Starbase software was utilized for prediction of the binding sites of TUG1 and microRNA-222-3p in addition to microRNA-222-3p and Smad2/7. Then dual-luciferase reporter gene assay was adopted to inspect the binding relationships between microRNA-222-3p and TUG1 or Smad2/7. Results Highly expressed TUG1 and lowly expressed microRNA-222-3p were found in human periodontal ligament stem cells during osteogenic differentiation. After measuring the expression of Runx2, ALP, and OCN as well as the formation of calcium nodules, we discovered that TUG1 can facilitate osteogenic differentiation of human periodontal ligament stem cells, while microRNA-222-3p had a reverse effect. Subsequently, knockdown of TUG1 and Smad2/7 or overexpression of microRNA-222-3p inhibited the osteogenic differentiation of human periodontal ligament stem cells. MicroRNA-222-3p is a target gene of TUG1, while microRNA-222-3p can negatively regulate Smad2/7. Conclusion LncRNA TUG1 accelerates the osteogenic differentiation of human periodontal ligament stem cells by sponging microRNA-222-3p to regulate Smad2/7. |
| Databáze: | OpenAIRE |
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