TNP-470 inhibits intraabdominal adhesion formation

Autor: Steven L. Moulton, John Kasznica, Karen S. Moulton, Christopher H. Cheng, Stephanie C. Chiang
Rok vydání: 2000
Předmět:
Zdroj: Journal of pediatric surgery. 35(2)
ISSN: 0022-3468
Popis: Background/Purpose: Angiogenesis plays an integral role in wound healing and tissue remodeling. The authors hypothesized that inhibition of angiogenesis would reduce intraabdominal adhesion formation. Methods: In 98 C57BL6/J mice, a 2-cm midline laparotomy was performed and a 5 mm 2 SILASTIC® (Dow Corning, Midland, MI) patch fixed to the right side of the peritoneum. Mice were injected with normal saline (n = 54) or TNP-470, an inhibitor of angiogenesis (n = 44; 30 mg/kg every other day over 6 days before surgery until 10 days after surgery). Animals were killed on postoperative days 10, 15, 35, and 55. Adhesions to the SILASTIC® (Dow Corning) patch were scored based on their extent, type, and tenacity. Angiogenesis was quantified digitally as the area of vascularized peritoneum over the patch. Results: At day 10, when TNP-470 was stopped, the percentage of vascularized peritoneum over the patch was less in treatment animals than in controls ( P =.004). At day 35, the patch in treatment animals was completely covered by vas-cularized peritoneum, similar to controls. Adhesions in TNP-470 animals were reduced at day 10 compared with controls ( P Conclusions: Angiogenesis appears to play an important role in the development of intraabdominal adhesions, because the extent of early neovascularization correlates with adhesion formation. Perioperative treatment with TNP-470, a potent endothelial cell inhibitor, reduced vessel ingrowth over the patch and was associated with a sustained reduction in adhesion formation. J Pediatr Surg 35:189-196. Copyright © 2000 by W.B. Saunders Company.
Databáze: OpenAIRE
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