Diagnostic accuracy of an IgM enzyme-linked immunosorbent assay and comparison with 2 polymerase chain reactions for early diagnosis of human leptospirosis
| Autor: | Yosena Chiani, María Fernanda Schmeling, C Cudos, H.D. Tarabla, Javier Lottersberger, N Landolt, P Jacob, Norma Bibiana Vanasco |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
DNA Bacterial Male 0301 basic medicine Microbiology (medical) 030106 microbiology Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Sensitivity and Specificity law.invention 03 medical and health sciences law Direct agglutination test medicine Humans Leptospirosis Polymerase Polymerase chain reaction Aged chemistry.chemical_classification 030505 public health biology business.industry Area under the curve General Medicine Middle Aged medicine.disease Antibodies Bacterial Virology Molecular biology Early Diagnosis Infectious Diseases Enzyme Real-time polymerase chain reaction Immunoglobulin M chemistry biology.protein Female 0305 other medical science business |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 84:292-297 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2016.01.002 |
| Popis: | Enzyme-linked immunosorbent assay (ELISA) tests and polymerase chain reaction (PCR) may play a key role for early detection and treatment of human leptospirosis in developing countries. The aims of this study were to develop and validate an IgM ELISA under field conditions and to compare the diagnostic accuracy among IgG, IgM ELISAs, conventional PCR (cPCR), and real-time PCR (rtPCR) for early detection of human leptospirosis. Overall accuracy of IgM ELISA was sensitivity of 87.9%, specificity of 97.0%, and area under the curve of 0.940. When the 4 methods were compared, IgM ELISA showed the greatest diagnostic accuracy (J=0.6) followed by rtPCR (J=0.4), cPCR (J=0.2) and IgG ELISA (J=0.1). Our results support the use of IgM ELISA and rtPCR for early diagnosis of the disease. Moreover, due to their high specificity, they could be also useful to replace or supplement microscopic agglutination test as a confirmatory test, allowing more confirmations. |
| Databáze: | OpenAIRE |
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