QTL analysis of the developmental response to L-glutamate in Arabidopsis roots and its genotype-by-environment interactions
Autor: | Brian G. Forde, Rhonda C. Meyer, Thomas Altmann, Pia Walch-Liu |
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Rok vydání: | 2017 |
Předmět: |
QTL mapping
temperature sensitivity 0106 biological sciences 0301 basic medicine root growth Genotype Physiology Quantitative Trait Loci Arabidopsis Glutamic Acid glutamate Locus (genetics) Plant Science root architecture Quantitative trait locus Plant Roots 01 natural sciences Chromosomes Plant Epigenesis Genetic 03 medical and health sciences Inbred strain nitrate natural variation skin and connective tissue diseases Gene Genetics Nitrates biology epistatic effects Temperature Glutamate receptor Environmental interactions Chromosome Mapping Genetic Variation biology.organism_classification 030104 developmental biology Chromosome 3 Epistasis Gene-Environment Interaction sense organs Research Paper 010606 plant biology & botany |
Zdroj: | Journal of Experimental Botany |
ISSN: | 1460-2431 0022-0957 |
Popis: | A major QTL controlling glutamate sensitivity of Arabidopsis root development has been mapped and additional loci identified that epistatically regulate its response to environmental changes. Primary root growth in Arabidopsis and a number of other species has previously been shown to be remarkably sensitive to the presence of external glutamate, with glutamate signalling eliciting major changes in root architecture. Using two recombinant inbred lines from reciprocal crosses between Arabidopsis accessions C24 and Col-0, we have identified one large-effect quantitative trait locus (QTL), GluS1, and two minor QTLs, GluS2 and GluS3, which together accounted for 41% of the phenotypic variance in glutamate sensitivity. The presence of the GluS1 locus on chromosome 3 was confirmed using a set of C24/Col-0 isogenic lines. GluS1 was mapped to an interval between genes At3g44830–At3g46880. When QTL mapping was repeated under a range of environmental conditions, including temperature, shading and nitrate supply, a strong genotype-by-environment interaction in the controls for the glutamate response was identified. Major differences in the loci controlling this trait were found under different environmental conditions. Here we present evidence for the existence of loci on chromosomes 1 and 5 epistatically controlling the response of the GluS1 locus to variations in ambient temperature, between 20°C and 26°C. In addition, a locus on the long arm of chromosome 1 was found to play a major role in controlling the ability of external nitrate signals to antagonize the glutamate effect. We conclude that there are multiple loci controlling natural variation in glutamate sensitivity in Arabidopsis roots and that epistatic interactions play an important role in modulating glutamate sensitivity in response to changes in environmental conditions. |
Databáze: | OpenAIRE |
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