Tangential Flow Filtration for the Concentration of Oncolytic Measles Virus: The Influence of Filter Properties and the Cell Culture Medium

Autor:	Tobias Weidner, Peter Czermak, Hauke Dieken, Denise Salzig, Daniel Loewe, Tanja A. Grein
Rok vydání:	2019
Předmět:	0106 biological sciences 0301 basic medicine cross-linked regenerated cellulose viruses Filtration and Separation 01 natural sciences Article Cross-flow filtration Measles virus 03 medical and health sciences serum-containing medium 010608 biotechnology Chemical Engineering (miscellaneous) oncolytic virus biology Fouling Chemistry membrane fouling Process Chemistry and Technology Membrane fouling mathematical modeling biology.organism_classification Blood proteins Oncolytic virus 030104 developmental biology Membrane Cell culture Biophysics polyether sulfone flat-sheet membrane serum-free medium
Zdroj:	Membranes Volume 9 Issue 12
ISSN:	2077-0375
Popis:	The therapeutic use of oncolytic measles virus (MV) for cancer treatment requires > 108 infectious MV particles per dose in a highly pure form. The concentration/purification of viruses is typically achieved by tangential flow filtration (TFF) but the efficiency of this process for the preparation of MV has not been tested in detail. We therefore investigated the influence of membrane material, feed composition, and pore size or molecular weight cut-off (MWCO) on the recovery of MV by TFF in concentration mode. We achieved the recovery of infectious MV particles using membranes with a MWCO &le 300 kDa regardless of the membrane material and whether or not serum was present in the feed. However, serum proteins in the medium affected membrane flux and promoted fouling. The severity of fouling was dependent on the membrane material, with the cellulose-based membrane showing the lowest susceptibility. We found that impurities such as proteins and host cell DNA were best depleted using membranes with a MWCO &ge 300 kDa. We conclude that TFF in concentration mode is a robust unit operation to concentrate infectious MV particles while depleting impurities such as non-infectious MV particles, proteins, and host cell DNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b864d8d7c5d70f2395355730e048f92 https://pubmed.ncbi.nlm.nih.gov/31795406 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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