Enhanced siRNA delivery using a combination of an arginine-grafted bioreducible polymer, ultrasound, and microbubbles in cancer cells
| Autor: | Sung Wan Kim, Stelios Florinas, Hye Yeong Nam |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Vascular Endothelial Growth Factor A
Polymers Genetic enhancement Pharmaceutical Science Biology Arginine Article chemistry.chemical_compound Drug Stability Cell Line Tumor Drug Discovery Gene Knockdown Techniques medicine Humans Ultrasonics RNA Small Interfering Ovarian Neoplasms Drug Carriers Microbubbles Gene Transfer Techniques Cancer Genetic Therapy medicine.disease Molecular biology Vascular endothelial growth factor Vascular endothelial growth factor A chemistry Cancer cell Cancer research Molecular Medicine Female Drug carrier |
| Popis: | RNAi-based gene therapy for cancer treatment has not shown significant clinical impact due to poor siRNA delivery to the target site. Here, we design a non-viral siRNA gene carrier using a combination of an arginine-grafted bioreducible polymer (ABP), microbubbles (MB), and ultrasound (US), for targeting vascular endothelial growth factor (VEGF) in a human ovarian cancer cell line. Newly designed MBs with a perfluorocrownether gas core show higher stability compared to controls. Further, MBs in combination with polyplexes show a significant higher loading capacity compared to naked siRNA. Lastly, only siRNA-ABP-MB (SAM) complexes in combination with US show significant VEGF knock down in A2780 human ovarian cancer cell line compared to naked siRNA when incubated for a short time after sonication treatment. |
| Databáze: | OpenAIRE |
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