Expression of Aspergillus hemoglobin domain activities in Aspergillus oryzae grown on solid substrates improves growth rate and enzyme production
| Autor: | Rob te Biesebeke, Amandine Boussier, Cees A. M. J. J. van den Hondel, Nick van Biezen, Willem M. de Vos, Machtelt Braaksma, Peter J. Punt |
|---|---|
| Přispěvatelé: | TNO Kwaliteit van Leven |
| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_treatment
Aspergillus oryzae Gene Expression Protein Engineering Applied Microbiology and Biotechnology Microbiology Hemoglobins Microbiologie Solid-state fermentation medicine Protein production Amylase Hemoglobin Biology VLAG Cell Proliferation chemistry.chemical_classification Aspergillus Protease biology Growth rate Aspergillus niger Wild type food and beverages General Medicine biology.organism_classification Recombinant Proteins Protein Structure Tertiary Oxygen Enzyme Genetic Enhancement Biochemistry chemistry Amylases biology.protein Molecular Medicine Glucan 1 4-alpha-Glucosidase Peptide Hydrolases Biotechnology |
| Zdroj: | Biotechnology Journal 1 (2006) 7-8 Biotechnology Journal, 7-8, 1, 822-827 Biotechnology Journal, 1(7-8), 822-827 |
| ISSN: | 1860-6768 |
| Popis: | DNA fragments coding for hemoglobin domains (HBD) were isolated from Aspergillus oryzae and Aspergillus niger. The HBD activities were expressed in A. oryzae by introduction of HBD gene fragments under the control of the promoter of the constitutively expressed gpdA gene. In the transformants, oxygen uptake was significantly higher, and during growth on solid substrates the developed biomass was at least 1.3 times higher than that of the untransformed wild-type strain. Growth rate of the HBD-activity-producing strains was also significantly higher compared to the wild type. During growth on solid cereal substrates, the amylase and protease activities in the extracts of the HBD-activity-producing strains were 30-150% higher and glucoamylase activities were at least 9 times higher compared to the wild-type strain. These results suggest that the Aspergillus HBD-encoding gene can be used in a self-cloning strategy to improve biomass yield and protein production of Aspergillus species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text