Changes in the gene expression profile of Arabidopsis thaliana under chromium stress
| Autor: | Jianxia Liu, Yonghong Han, Zikuan Gai, Weihao Li, Guotao Ding, Wei Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Potassium Compounds
Health Toxicology and Mutagenesis 0211 other engineering and technologies Sulfur metabolism Arabidopsis 02 engineering and technology 010501 environmental sciences 01 natural sciences chemistry.chemical_compound Downregulation and upregulation Chromium Compounds Gene expression Chromates Arabidopsis thaliana Photosynthesis Secondary metabolism Gene Abscisic acid 0105 earth and related environmental sciences 021110 strategic defence & security studies Nitrates biology Indoleacetic Acids Chemistry Public Health Environmental and Occupational Health General Medicine biology.organism_classification Microarray Analysis Pollution Molecular biology Energy Metabolism Transcriptome Oxidation-Reduction Abscisic Acid |
| Zdroj: | Ecotoxicology and environmental safety. 193 |
| ISSN: | 1090-2414 |
| Popis: | Based on previous studies and preliminary test results, 200 μM was used as the test concentration of chromium (Cr), and changes in the gene expression profile of Arabidopsis thaliana in response to 24-h treatments of Cr(III) and Cr(VI) were analyzed using the Arabidopsis ATH1 Genome Array. The results were as follows. There were 238 upregulated genes and 858 downregulated genes in response to treatments with Cr(III) and Cr(VI). For Cr(III) and Cr(VI) treatments, there were 185 and 587 specifically upregulated genes as well as 220 and 956 specifically downregulated genes, respectively. Among the common differentially expressed genes (DEGs), the expression levels of genes involved in redox, secondary metabolism, and energy metabolism processes were significantly downregulated, while those of genes related to the stress response, photosynthesis, and sulfur metabolism were significantly upregulated. These findings indicated that Cr seriously affected the normal activities of A. thaliana cells. Some genes associated with stress and regulation were upregulated to adapt to the stress caused by Cr. Among the unique DEGs, the expression levels of genes involved in indole-3-acetic acid (IAA) regulatory pathway were significantly increased in response to Cr(III) treatment; the expression levels of genes involved in the abscisic acid (ABA) regulation pathway and carotenoid synthesis were significantly increased following Cr(VI) treatment. These results revealed some differences in response to Cr(III) and Cr(VI) in A. thaliana. |
| Databáze: | OpenAIRE |
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