Kisspeptin-10 and the G protein-coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells
| Autor: | HB Beceriklisoy, Mahmut Sözmen, Murat Findik, Selim Aslan, Ali Reha Ağaoğlu, T.W. van Haeften, Serhan Serhat Ay, Duygu Kaya, Sabine Schäfer-Somi |
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| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty GPR54 placenta Ovariectomy media_common.quotation_subject Uterus kiss1 Biology Hysterectomy Receptors G-Protein-Coupled 03 medical and health sciences Dogs 0302 clinical medicine Endocrinology Syncytiotrophoblast Downregulation and upregulation Pregnancy Internal medicine Placenta medicine Animals Receptor Ovulation Progesterone myocyte media_common Kisspeptins 030219 obstetrics & reproductive medicine Trophoblast Immunohistochemistry Trophoblasts 030104 developmental biology medicine.anatomical_structure Pregnancy Animal Female Animal Science and Zoology domestic dog (Canis familiaris) hormones hormone substitutes and hormone antagonists Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 52:123-129 |
| ISSN: | 0936-6768 |
| DOI: | 10.1111/rda.12818 |
| Popis: | 8th International Symposium on Canine and Feline Reproduction (ISCFR) / 19th Congress of the European-Veterinary-Society-for-Small-Animal-Reproduction (EVSSAR) -- JUN 22-25, 2016 -- Paris, FRANCE FINDIK, Murat/0000-0003-1408-2548 WOS: 000399379200022 PubMed: 27774662 Contents Uterine tissue was collected from bitches after ovariohysterectomy at different times after ovulation. Samples were assigned to four groups: metestrous non-pregnant, day 10-12, n=4; pre-implantation, day 10-12, n=9; post-implantation, day 18-25, n=13; mid-gestation, day 30-40, n=7. RT-qPCR detection was performed for kiss1 and the G protein-coupled receptor 54 (GPR54, specific receptor for kisspeptin). In addition, immunohistochemistry was performed for detection of kisspeptin-10 (KP-10), GPR54, as well as pan-cytokeratin and vimentin. The latter two were included to differentiate the different placental cell types. The percentage of positive stained cells was evaluated, and an immunoreactivity score (IRS) was obtained by multiplying the labelling intensity score (0-3) with the percentage of immunolabelled cells (range: 0-300). In non-pregnant and pre-implantation tissues, gene expression was highly variable for kiss1 and GPR54. Expression of GPR54 was higher before embryo adhesion than during post-implantation and mid-gestation (p |
| Databáze: | OpenAIRE |
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