Isolation and characterization of a novel antihyperglycemic protein from the fruits of Momordica cymbalaria
| Autor: | Maddirela Dilip, Rajasekhar, Kameswara Rao, Badri, Kondeti, Vinay Kumar, Kasetti Ramesh, Babu, Ramesh Babu, Kassetti, Shaik Sameena, Fatima, Mekala Thur, Sampath Kumar, Chippada, Appa Rao |
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| Rok vydání: | 2008 |
| Předmět: |
Size-exclusion chromatography
Pharmacognosy Mass Spectrometry Streptozocin Diabetes Mellitus Experimental Column chromatography Drug Discovery Animals Hypoglycemic Agents Momordica Peptide sequence Chromatography High Pressure Liquid Plant Proteins Pharmacology biology Chemistry biology.organism_classification Rats Isoelectric point Biochemistry Sephadex Chromatography Gel Momordica cymbalaria Electrophoresis Polyacrylamide Gel Isoelectric Focusing |
| Zdroj: | Journal of ethnopharmacology. 128(1) |
| ISSN: | 1872-7573 |
| Popis: | Aim of the study A new antihyperglycemic protein was identified in the aqueous extract of fruits of Momordica cymbalaria by bioassay-guided fractionation. The study was aimed at isolation and characterization of this protein. Materials and methods The active principle was purified to homogeneity by ammonium sulphate precipitation, gel filtration column chromatography on Sephadex G-50 followed by reverse phase HPLC. Its N-terminal amino acid sequence was identified and compared in the protein data bank. Optimum dose and route of administration of the active principle was determined in STZ induced diabetic rats. Results A 17 kDa protein with an isoelectric point of 5.0 was identified as the active principle of antidiabetic action present in the aqueous extract of fruits of MC. It is named as M.Cy protein and found to be a novel protein by comparing its N-terminal amino acid sequence with those in the protein data bank. It did not produce any hypoglycemia in either normal or diabetic rats. Conclusions The results suggest that ‘M.Cy protein’, present in the fruits of Momordica cymbalaria is an effective antihyperglycemic active principle in STZ induced diabetic rats at a dose of 2.5 mg/kg b.w. |
| Databáze: | OpenAIRE |
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