Dual lateral flow optical/chemiluminescence immunosensors for the rapid detection of salivary and serum IgA in patients with COVID-19 disease
| Autor: | Laura Anfossi, Aldo Roda, Sergio Rosati, Fabio Di Nardo, Patrizia Simoni, Barbara Colitti, Donato Calabria, Claudio Baggiani, Simone Cavalera, Matilde Roda |
|---|---|
| Přispěvatelé: | Roda A., Cavalera S., Di Nardo F., Calabria D., Rosati S., Simoni P., Colitti B., Baggiani C., Roda M., Anfossi L. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Immunoglobulin A
Saliva Chemiluminescence Biomedical Engineering Biophysics Context (language use) 02 engineering and technology Biosensing Techniques Antibodies Viral 01 natural sciences Article Luminol law.invention Serology COVID-19 Serological Testing chemistry.chemical_compound Biosensing Technique Antigen law Antibody Specificity Electrochemistry Medicine Humans biology Rapid serological tests business.industry SARS-CoV-2 010401 analytical chemistry Rapid serological test COVID-19 General Medicine Equipment Design 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Luminescent Measurement Immunology Luminescent Measurements Immunoglobulin A Secretory biology.protein Colorimetry Antibody 0210 nano-technology business Biotechnology Human |
| Zdroj: | Biosensors and Bioelectronics Biosensors & Bioelectronics |
| Popis: | To accurately diagnose COVID-19 infection and its time-dependent progression, the rapid, sensitive, and noninvasive determination of immunoglobulins A specific to SARS-CoV-2 (IgA) in saliva and serum is needed to complement tests that detect immunoglobulins G and M. We have developed a dual optical/chemiluminescence format of a lateral flow immunoassay (LFIA) immunosensor for IgA in serum and saliva. A recombinant nucleocapsid antigen specifically captures SARS-CoV-2 antibodies in patient specimens. A labelled anti-human IgA reveals the bound IgA fraction. A dual colorimetric and chemiluminescence detection enables the affordable and ultrasensitive determination of IgA to SARS-CoV-2. Specifically, a simple smartphone-camera-based device measures the colour signal provided by nanogold-labelled anti-human IgA. For the ultrasensitive chemiluminescence transduction, we used a contact imaging portable device based on cooled CCD, and measured the light signal resulting from the reaction of the HRP-labelled anti-human IgA with a H2O2/luminol/enhancers substrate. A total of 25 serum and 9 saliva samples from infected and/or recovered individuals were analysed by the colorimetric LFIA, which was sensitive and reproducible enough for the semi-quantification of IgA in subjects with a strong serological response and in the early stage of COVID-19 infection. Switching to CL detection, the same immunosensor exhibited higher detection capability, revealing the presence of salivary IgA in infected individuals. For the patients included in the study (n = 4), the level of salivary IgA correlated with the time elapsed from diagnosis and with the severity of the disease. This IgA-LFIA immunosensor could be useful for noninvasively monitoring early immune responses to COVID-19 and for investigating the diagnostic/prognostic utility of salivary IgA in the context of large-scale screening to assess the efficacy of SARS-CoV-2 vaccines. Highlights • A LFIA for the point-of-care detection of IgA specific to SARS-CoV2 in serum and saliva was developed. • GNP and HRP-mediated optical/chemiluminescence signals were detected by a smartphone CMOS and a portable CCD, respectively. • The one-step colorimetric and two-step CL IgA-LFIA enabled the detection of anti-SARS-CoV-2 IgA in 15 min. • Salivary IgA were identified in four COVID-19 patients by the CL IgA-LFIA after two weeks from diagnosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|