Aqueous extract from Orthosiphon stamineus leaves prevents bladder and kidney infection in mice
| Autor: | M. R. Asadi Karam, S Sarshar, Francisco M. Goycoolea, Ulrich Dobrindt, Matthias Lechtenberg, Mehri Habibi, X Qin, Andreas Hensel, Simone Brandt, Saeid Bouzari |
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| Rok vydání: | 2017 |
| Předmět: |
Pharmaceutical Science
Motility Biology urologic and male genital diseases medicine.disease_cause Microbiology law.invention Mice In vivo law Drug Discovery medicine Animals Humans Uropathogenic Escherichia coli Orthosiphon Escherichia coli Escherichia coli Infections Norfloxacin Pharmacology Mice Inbred BALB C Kidney Plant Extracts Urinary Bladder Diseases Orthosiphon stamineus Epithelial Cells biology.organism_classification Anti-Bacterial Agents Plant Leaves Disease Models Animal Quorum sensing medicine.anatomical_structure Complementary and alternative medicine Urinary Tract Infections Molecular Medicine Female Kidney Diseases Phytotherapy medicine.drug |
| Zdroj: | Phytomedicine. 28:1-9 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2017.02.009 |
| Popis: | Background Extracts from the leaves of Orthosiphon stamineus are used in phytotherapy for treatment of uncomplicated urinary tract infections. Purposes Evaluation of an aqueous extract against infection with uropathogenic Escherichia coli in vivo; investigation of underlying microbiological mechanisms. Study design In vivo studies in mice and in vitro investigations on cytotoxicity, antiadhesive potential, influence on bacterial gene expression and quorum sensing. Methods Extract OWE was prepared by hot water extraction. For in vivo studies BALB/c mice were used in an UPEC infection model. The effect of OWE on bacterial load in bladder/kidney tissue was monitored in pre- and posttreatment. Cytotoxicity of OWE against different UPEC strains, T24 bladder/A498 kidney cells, gene expression analysis, monitoring of phenotypic motility and quorum sensing was investigated by standard methods of microbiology. Results OWE was quantified (UHPLC) according to the content of rosmarinic acid, cichoric acid, caffeic acid. Three- and 5-day treatment of animals with OWE (750 mg/kg) after transurethral infection with UPEC CFT073 reduced the bacterial load in bladder and kidney, similar to norfloxacin. Four- and 7-day pretreatment of mice prior to the infection with UPEC NU14 reduced bacterial bladder colonization. In vitro investigations indicated that OWE (≤2 mg/ml) has no cytotoxic or proliferation-inhibiting activity against different UPEC strains as well as against T24 bladder and A498 kidney cells. OWE exerts a dose dependent antiadhesive activity against UPEC strains NU14 and UTI89. OWE reduced gene expression of fimH, but evoked increase of the expression of motility/fitness gene fliC. Increase of bacterial motility on gene level was confirmed by a changed bacterial phenotype by an increased bacterial motility in soft agar assay. OWE inhibited in a concentration-dependent manner bacterial quorum sensing. Conclusion OWE is assessed as a strong antiadhesive plant extract for which the traditional use in phytotherapy for UTI might be justified. |
| Databáze: | OpenAIRE |
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