Heterologous Expression of the Avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae
| Autor: | Colin M. Lazarus, Zhongshu Song, Marc-Henri Lebrun, Elizabeth Skellam, Russell J. Cox, Walid Bakeer, Jérôme Collemare, Thomas J. Simpson, Rozida Mohd. Khalid, James W. Marshall, Didier Tharreau, Andy M. Bailey, Ahmed A. Yakasai |
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| Přispěvatelé: | Institut de Recherche en Horticulture et Semences (IRHS), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
Magnaporthe chaetoglobosin diaporthichalasin Oryza sativa Relation hôte pathogène F30 - Génétique et amélioration des plantes Interactions biologiques Aspergillus oryzae Gene cluster Expression des gènes phomopsichalasin [CHIM]Chemical Sciences synthase-peptide synthetase cluster Gene H20 - Maladies des plantes Genetics Appressorium biology Intron grisea food and beverages General Chemistry biology.organism_classification Résistance aux maladies proteins cytochalasin Biochemistry ddc:540 identification Heterologous expression biosynthesis Avirulence |
| Zdroj: | Chemical Science Chemical Science, The Royal Society of Chemistry, 2015, pp.4837-4845. ⟨10.1039/C4SC03707C⟩ Chemical Science, The Royal Society of Chemistry, 2015, 6 (8), pp.4837-4845. ⟨10.1039/c4sc03707c⟩ Song, Z, Bakeer, W I M M, Marshall, J W, Yakasai, A A, Mohd Khalid, R, Collemare, J, Skellam, E, Tharreau, D, Lebrun, M H, Lazarus, C M, Bailey, A M, Simpson, T J & Cox, R J 2015, ' Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae ', Chemical Science, vol. 6, no. 8, pp. 4837-4845 . https://doi.org/10.1039/c4sc03707c Chemical Science 6 (2015), Nr. 8 Chemical Science 8 (6), 4837-4845. (2015) |
| ISSN: | 2041-6520 2041-6539 |
| DOI: | 10.1039/C4SC03707C⟩ |
| Popis: | The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound. Government of Egypt Scholarship The School of Chemistry at the University of Bristol and the Mark Evans Scholarship Kano State Government Nigeria MacArthur Foundation Bayero University Nigerian Petroleum Technology Fund Malaysian Govenment Scholarship EP/F066104/1 |
| Databáze: | OpenAIRE |
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