Testicular subcutaneous allografting followed by immunosuppressive treatment promotes maintenance of spermatogonial cells in rainbow trout (Oncorhynchus mykiss)

Autor: Francisco Javier Hernandez-Blazquez, Pedro Kastein Faria da Cunha Bianchi, Ricardo Shohei Hattori, Cristina de Oliveira Massoco Salles Gomes, Arno Juliano Butzge, Ana Claudia Oliveira Carreira, Tulio Teruo Yoshinaga, José Roberto Kfoury Junior, Yara Aiko Tabata, Rennan Lopes Olio
Přispěvatelé: Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), UPD CJ APTA SAA
Rok vydání: 2019
Předmět:
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1095-9947
Popis: Made available in DSpace on 2021-06-25T15:01:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Germ cell transplantation and testis graft represent promising biotechnologies that can be applied for the reproduction of commercial or endangered species. However, mechanisms of rejection from the host immune system might remove the transplanted donor cells/tissues and limit the surrogate production of gametes. In this work, we administered emulsion containing-immunosuppressants to verify whether they are capable to prevent immune rejection and promote survival of testis allografts in rainbow trout. In the first part of this study, we demonstrated in vitro that tacrolimus and cyclosporine were able to affect viability, inhibit leucocyte proliferation, and suppress il2 expression in vitro. In in vivo experiments, both doses of tacrolimus (0.5 and 1.5 mg/kg) and the lower dose of cyclosporine (20 mg/kg) significantly inhibited the expression of il2 in head kidney, three days post-injection. A higher dose of cyclosporine (40 mg/kg) was able to inhibit il2 expression for up to seven days post-injection. In the second part, testis allografts were conducted in fish treated weekly with emulsion containing-tacrolimus. Immunohistochemical, conventional histology, and qRT-PCR (vasa) analysis demonstrated the presence of spermatogonial cells by the fifth week, in animals treated with 0.5 mg/kg of tacrolimus similar as found in autografted group. In the group treated with the highest tacrolimus dose (1.5 mg/kg) and in the non-treated group (without immunosuppressant), no germ cells or their respective markers were detected. il2 expression in head kidney was also suppressed in grafted animals treated with tacrolimus compared to nontreated group. These results suggest that tacrolimus may be a promising immunosuppressant for testis allografts or germ cell transplantation in rainbow trout. Co-administration combining tacrolimus (at lower dose) with other immunosuppressive drugs for inhibiting other activation pathways of the immune system, as performed in human organ transplantation, could be an alternative approach to optimize the immunosuppressive effects in host organisms. Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, BR-05508270 Sao Paulo, Brazil Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, BR-18618689 Botucatu, SP, Brazil Univ Sao Paulo, Biomed Sci Inst, Interunit Biotechnol Program, BR-05508900 Sao Paulo, Brazil Univ Sao Paulo, Cell & Mol Therapy Ctr, Sch Med, Internal Med Dept,NUCEL, BR-05360130 Sao Paulo, Brazil Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, BR-05508270 Sao Paulo, Brazil UPD CJ APTA SAA, Salmonid Expt Stn Campos do Jordao, BR-12460000 Campos Do Jordao, Brazil Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, BR-18618689 Botucatu, SP, Brazil FAPESP: 2013/17612-9 FAPESP: 2015/20762-8 CNPq: 404583/2016-4 CAPES: 001
Databáze: OpenAIRE
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