Accelerated Electro-Fermentation of Acetoin in Escherichia coli by Identifying Physiological Limitations of the Electron Transfer Kinetics and the Central Metabolism
| Autor: | Laura-Alina Philipp, Johannes Gescher, Sebastian Beblawy |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Life sciences
biology 0106 biological sciences Microbiology (medical) medicine.disease_cause 01 natural sciences Microbiology Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Electron transfer bulk chemicals ddc:570 010608 biotechnology Virology Escherichia coli medicine lcsh:QH301-705.5 030304 developmental biology chemistry.chemical_classification 0303 health sciences Acetoin Biowissenschaften Biologie [570] food and beverages Electron acceptor Electron transport chain lcsh:Biology (General) chemistry Biochemistry Yield (chemistry) methylene blue Fermentation metabolic engineering electro-fermentation acetoin |
| Zdroj: | Microorganisms, Vol 8, Iss 1843, p 1843 (2020) Microorganisms Volume 8 Issue 11 Microorganisms, 8 (11), Art.-Nr.: 1843 Microorganisms 8 (11): 1843 (2020-11) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8111843 |
| Popis: | Anode-assisted fermentations offer the benefit of an anoxic fermentation routine that can be applied to produce end-products with an oxidation state independent from the substrate. The whole cell biocatalyst transfers the surplus of electrons to an electrode that can be used as a non-depletable electron acceptor. So far, anode-assisted fermentations were shown to provide high carbon efficiencies but low space-time yields. This study aimed at increasing space-time yields of an Escherichia coli-based anode-assisted fermentation of glucose to acetoin. The experiments build on an obligate respiratory strain, that was advanced using selective adaptation and targeted strain development. Several transfers under respiratory conditions led to point mutations in the pfl, aceF and rpoC gene. These mutations increased anoxic growth by three-fold. Furthermore, overexpression of genes encoding a synthetic electron transport chain to methylene blue increased the electron transfer rate by 2.45-fold. Overall, these measures and a medium optimization increased the space-time yield in an electrode-assisted fermentation by 3.6-fold. Bundesministerium für Bildung und Forschung |
| Databáze: | OpenAIRE |
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