A significantly non-toxic novel Cobalt(III) Schiff base complex induces apoptosis via G2-M cell cycle arrest in human breast cancer cell line MCF-7
| Autor: | Sanchari Dasgupta, Kanisha Kar, Atish Barua, Diya Ghosh, Bikash Kabi, Koushik Dewan, Arpita Chandra |
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| Rok vydání: | 2022 |
| Předmět: |
Cell Cycle
Antineoplastic Agents Apoptosis Breast Neoplasms Cell Cycle Checkpoints Cobalt General Medicine General Biochemistry Genetics and Molecular Biology Oxaliplatin Mice Coordination Complexes Cell Line Tumor MCF-7 Cells Animals Humans Female Annexin A5 Cisplatin General Pharmacology Toxicology and Pharmaceutics Schiff Bases Cell Proliferation |
| Zdroj: | Life Sciences. 308:120963 |
| ISSN: | 0024-3205 |
| Popis: | Metal complexes have ignited considerable interest in the field of chemotherapy after the serendipitous discovery of cisplatin but the severe toxicity of these platinum-based drugs compelled researchers to search for newer, more effective lesser toxic anticancer drugs.Structural analysis is done by different physicochemical techniques including X-ray single crystallography. Toxicity study has been done in normal Swiss albino mice. MTT assay assessed cell viability. Apoptosis, cell cycle arrest, and cell proliferation were assessed by FACS using Annexin V-PI, PI, and CFSE staining respectively. Western blot quantifies protein expression. While cell migration was studied by wound healing assay.One-pot synthesis of a novel mononuclear cobalt(III)-Schiff base complex (1) (99 % purity) and its complete characterization have been done. Cell viability assay showed that 1 (ICThe readily synthesizable, significantly nontoxic cobalt complex with appreciable anticancer activity implies that it might be an effective chemotherapeutic agent for new-age anti-tumor medication. |
| Databáze: | OpenAIRE |
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